Expression And Function Study Of The Long Non-coding RNA RNANONHSAT070806 In Bladder Cancer

Backround Bladder cancer is the most common malignancy of the urinary tract.Tumor invasion plays an important role in the process of its development. According to the difference in the level of tumor invasion, bladder cancer can be devided into two types: muscle-invasive bladder cancer (MIBC) and non-muscle-invasive bladder cancer(NMIBC). MIBC is negatively associated with patient prognosis and the mortality is significantly higher than NMIBC. In recent years, although the relevant diagnostic methods have greatly improved, the clinical diagnosis of bladder cancer still rely on bladder microscopy, that causes adverse impact to the early screening for bladder cancer,therefore, in-depth exploration of its etiology mechanisms is very important. Long noncoding RNAs (lncRNAs) are RNAs that contain more than 200 nucleotides but do not have a protein coding region,own 80 percent of the total RNAs in human. Recent studies have shown that lncRNAs play an important role in the occurrence and development of malignant tumours, including gastric cancer, prostate cancer, breast cancer, lung cancer and bladder cancer. However, the related mechanism research is insufficient.Methords The LncRNAs expression of bladder cancer tissue and adjacent normal tissue are detected using gene chip assay. The LncRNAs with great different expression in cancer tissue compared with adjacent tissue are selected and verified in 34 bladder cancer patients using qPCR. The correlation of these LncRNAs with the clinical features of bladder cancer patients are analyzed. Then we establishe a NONHSAT070806 over-expression vector and transfect it into bladder cancer cells T24 and 5637, then detect the change of cell apoptosis, proliferation,invasion,migration and in-vitrotumourigenesis after transfection, in order to explore the in-vitro effects of NONHSAT070806 on bladder cancer cells. Meanwhile, we both translate the over-expression vector and nagetive-control vector in the subcutaneous of the same nude mouse to build a xenotransplanted tumor model. Then we analyze the in-vivo effects of NONHSAT070806 on the tumor capacity of bladder cancer cells through measure the volume and size of the tumor in different group.Results A sense LncRNA NONHAST070806 is found to downregulate in bladder cancer patients (24/34, 70.6%) , and its expression level is negative relate to the degree of tumor invasion (p<0.05), while it has no statistical correlation with other clinical features like age, gender and smoking history. Moreover, ectopic expression of NONHAST070806 in T24 and 5637 bladder cancer cells induce apoptosis and promote cell growth, migration, invasion and in-vitro tumourigenesis (p<0.05) . Additionally,overexpression of NONHAST070806 inhibite tumourigenesis in vivo (p<0.05).Conclusion NONHAST070806 is downregulated in bladder cancer, and its expression level is negative relate to the degree of tumor invasion, may play a role as the tumor-suppressor of bladder cancer through inhibiting tumor invasion. The next step research about its mechanism could help to reveal the pathogenesis of bladder cancer.