| Background and Objective Burn is a common accident trauma at home and abroad while the superb medical technology level has greatly reduced the mortality of burn patients,but also burn patients prone to stress lasting and strong,which leads to some stress injury related system.Among them,the brain,as the advanced nerve center that produces the sensation,the control movement,and completes each kind of advanced brain function,the damage caused by the stress response is particularly prominent.At present,there are few studies on the animal model of severe burn injury.The research is based on the model of early brain injury in burn rat,and applied T2 WI,DWI and DTI,combined with pathological examination,through the detection at different time points(3H,6h,12 h,18h,24 h,48h,72h)early stress-induced brain injury in animal models of brain tissue characteristics of severe burns,to clarify the early burn brain damage imaging characteristics,and the results were compared with pathological study.Materials and methods the experiment selected 40 male SD rats(280±31.8g),were randomly divided into normal control group and different time points(3H,6h,12 h,18h,24 h,48h,72h)burn model group,each group of 5 rats.The model of burn stress was established in conscious rats.using magnetic resonance(German SIEMENS Skyra 3.0superconducting MR scanner)and the brain coil(Shanghai Chenguang Medical Technology Co Ltd)at different time points after the burn,T2 WI sequence,DWI sequence and DTI sequence scanning.By using SIEMENS company's Syngo.via software,to observe the image sequence of T2WI?DWI and DTI,and post processing,measured at different time points in different parts of rat brain(bilateral parietal cortex,frontal cortex,caudate nucleus)of T2 WI SI,ADC and FA values.The rats in each group were subjected to magnetic resonance imaging and the brain tissue was rapidly taken under anesthesia,pathological examination was made on the corresponding site.Then the whole data were tested for normal distribution,after the normal distribution was confirmed,analysis andcomparison between groups using one-way ANOVA,independent sample t test was performed on the measured values by SPSS22.0 statistical software,compared the different parts of the T2 WI SI value,FA value and ADC value at different time points on whether there is significant difference.P<0.05 is statistically significant,and the imaging results were compared with pathology.Results1.There was no statistical difference in T2 WI SI value,FA value and ADC value of different parts between different parts of the cerebral hemispheres.2.Different parts of the ADC value :compared with the different parts of the burn group and the control group,there was no significant difference in the ADC value of 3hours group,the ADC value of 6 hours group was lower than the control group,reached the lowest value after 24 hours,the ADC value of 48 hours group and 72 hours group were increased,but still lower than that of control group;The ADC value of caudate nucleus was found to be statistically significant after 12 hours,and the change trend was similar to that of the frontal and parietal cortex,and the difference was statistically significant.3.Different parts of the T2 WI SI value: compared with the control group and the burn group,there was no obvious difference in the T2 WI SI value of each part of 3 hours to 12 hours group,18 hours group and the group of 24 hours had an upward trend,and there was a downward trend in the group of 48 hours and 72 hours,but still higher than the normal group,the difference was statistically significant.4.Different parts of the FA value: compared with the control group and the burn group,There was no significant difference in the FA values of the caudate nucleus after burn.the frontal and parietal cortex after burn in 12 hours was lower than that in the control group,and reached the lowest value after 24 hours,then gradually increased,but still lower than the normal control group,the difference was statistically significant.5.HE staining: in addition to the nerve and blood vessels around of the control group have some gaps,the morphology of nerve cells is normal,the distribution is uniform,the number is large and the arrangement is regular.There was no obvious swelling in glial cells.the 6 hour group were mild swelling,perivascular space widened slightly,with prolonged time after burn,the lesions increased and aggravated in varying degrees,occasionally focal neurons were necrotic,edema changing obviously in brain tissues.the swelling degree of 48 hours and 72 hours group was decreased compared with that in the 24 hour group.conclusion Magnetic resonance DWI,DTI and T2 WI techniques combined with pathological examination can be used to evaluate the dynamic changes of brain injury in rats with severe burn injury,it can provide theoretical basis and evidence based support for early diagnosis,intervention and treatment of early stress brain injury after burn. |
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