MiR-138-5p Inhibits Migration Of Tongue Cancer Through Targeting DEK

Objective To elucidate the biological function of miR-138-5p / DEK and explore the mechanism of miR-138-5p targeting DEK to inhibit the migration of tongue cancer cells.Methods Immunohistochemistry was used to study the relationship between DEK expression and regional lymph node metastasis;To construct the stable clones of overexpression and knockdown DEK of tongue squamous cell carcinoma SCC6 and SCC15 cells,the scratch experiments and Transwell experiments were carried out to study the effect of overexpression and knockdown DEK on cell migration;Cyclin-A protein was detected in these stable clone cells,to study the correlation between DEK expression and Cyclin-A expression;The miRNAs targeting DEK were screened and miRNA which controlled the DEK gene were chosed,that was miR-138-5p;The miR-138-5p mimic / inhibitor were used to perform qRT-PCR,to clarify that this regulation was at the transcriptional level or post-transcriptional level;Construction of DEK WT and DEK MUT for transcriptional activity experiments to verify that mi R-138-5p regulated the expression of DEK protein and its downstream genes by down-regulating the activity of DEK 3'UTR;Collecting tongue cancer specimens,the expression of mi RNA-138-5p in tongue squamous cell carcinoma was detected by situ hybridization;MiR-138-5p and its inhibitor were transfected into tongue cancer cells,the effects of miR-138-5p on cell migration were examined by scratches and Transwell.Further study about the mechanism,in different cell lines,Western blot was used to study the specific mechanism of miR-138-5p / DEK on the migration of tongue cancer.Results Results of immunohistochemistry showed that DEK was highly expressed in tongue cancer tissues with regional lymph node metastasis,DEK protein promoted the metastasis of cancer cells;In SCC6 and SCC15 cells,Cyclin-A was highly expressed in PCDH-DEK cells and low in PSIH-DEK;Mi R-138-5p could regulate the expression of DEK,mRNA level of DEK was significantly decreased when miR-138-5p was overexpressed,it indicated that miR-138-5p regulated the expression of DEK protein mainly at the transcriptional level;Mi R-138-5p regulated the expression of DEK protein and its downstream genes by down-regulating the activity of DEK 3'UTR;In tongue cancer,the results of situ hybridization found that the expression level of mi R-138-5p was low in tongue squamous cell carcinoma with regional lymph node metastasis,it was negatively correlated with DEK expression;MiR-138-5p was found to inhibit the migration of tongue cancer cells;DEK through the EMT pathway to promote the migration of tongue cancer cells,the effect of miR-138-5p on the migration of tongue cancer was dependent on DEK,and it could regulate the downstream gene of DEK.Conclusion Mi RNA-138-5p can be seen as a tumor suppressor gene in tongue cancer,it affects the migration of tongue cancer cells through the role of DEK.DEK can regulates the migration of tongue cancer cells and plays an important role in the transfer of tongue cancer.We confirmed the inhibitory effect of miR-138-5p on the migration of tongue cancer cells,elucidated the mechanism.The study of miR-138-5p / DEK enriches the theory of the development of tongue cancer and will be expected to bring new hopes to the biological therapy of tongue cancer.