Expression And Significance Of Autophagy-related Gene In Chronic Non-bacterial Prostatitis Rat Model

Objective To explore the possible role of autophagy in chronic prostatitis by exploring the level of autophagy-related gene autophagy microtubule associated protein 1 light chain 3(LC3)and Beclin-1 expression in chro nic non-bacterial prostatitis rat model.Methods(1)6 male Sprague-Dawley(SD)rats weighing 300 g were cut into the prostate tissue under sterile condition.The scissors were cut and homogenized in the glass homogenizer.After homogenization,the cells were centrifuged in a frozen centrifuge,12 000 r / min,4 ?,30 min,the supernatant was taken and the protein was diluted with BCA to 40 g / L;(2)40 rats of 3-month-old male SD rats were randomly divided into normal control group,2-week CNP model group,4-week CNP model group,6-week CNP model group,10 rats in each group,CNP model group using prostate antigen in situ injection,Each rat injected 0.2ml prostate protein purification solution,the control group in situ injection of the same amount of saline;(3)hematoxylin-eosin(HE)staining was used to observe the infiltration of inflammatory cells in each group;(4)The expression of LC3 and Beclin-1 in prostate tissue were detected by SABC(strept avidin-biotin complex)immunohistochemistry;(5)Western blot was used to detect the expression of LC3-? / ? and the expression of Beclin-1 in prostate tissue;(6)SPSS 16.0 software was used for statistical analysis.The measurement data is described by (?)±s,Multiple-group comparisons were performed using One-Way ANOVA analysis.The SNK-q test was used for the comparison.The test level was ? = 0.05.Results(1)Successful extract prostate protein purification solution to a concentration of 40 g / L;(2)HE staining results suggested that the model group successfully constructed chronic nonbacterial prostatitis in rats,the normal control group no obvious inflammation.The infiltration rate of the 6 week model group was higher than that of the 4 week model group and the 2 week model group;(3)Immunohistochemical method showed that the expressio n of LC3 and Beclin-1 protein in each experimental group was higher than that in the control group and gradually increased with the passage of time,the difference was statistically significant(P<0.05).(4)Western blot was used to detect the level of LC3-? / ? in the model group,which was significantly higher than that in the normal control group(P <0.05).There was no significant difference in LC3-? / ? transformation between the 2-week model group and the 4-week model group.The conversion level of LC3-? / ? in the 6-week model group was higher than that in the 2-week model group and the 4-week model group,the difference was statistically significant(P <0.05).Conclusion The in vitro injection of prostatic protein purification solution can construct SD rats with chronic nonbacterial prostatitis;Autophagy-related genes LC3 and Beclin-1 were highly expressed in the pathogenesis of chronic nonbacterial prostatitis rat models.