| Objective:To promote the biological responses of the implant via nanostructured surface design is essential for osteoblast cell growth and osseointegration.Method:Rutile TiO2 nanorod films have been prepared on 3D-printed titanium substrate via a hydrothermal method using Tetra-n-butyl titanate as Ti source in the presence of concentrated hydrochloric acid.The surface topography in nanoscale has been studied using scanning electron microscopy(SEM).The fluorescent images revealed that the cells attached and proliferated condition on all three surfaces-Titanium foils(Ti);3D-printed Titanium foils after surface acid etching(3D+Acid)and TiO2 nanorods integrated onto 3D-printed titanium substrates(3D+Nano)-after 1,3,5d incubation ofBMSCs of SD rat.After 7,14d,expression of osteoblast specific genes,were confirmed by RT-PCR.Result:The surface topological structure of Ti group was smooth,3D+Acid group has roughness in micron level,while 3D+Nano has a nano-micro composite structure.After 24h incubation of BMSCs,the fluorescence microscope suggested that the quantity of attached cells on Ti group was the most,while the area of cell spread was the best on 3D+Nano group;The fluorescent images showed the proliferation of cells was enhanced on 3D+Nano group comparing to other two groups;the results of cck-8 examine complemented that.3D+Acid group and 3D+Nano group have a higher expression on Col-1,OCN,RUNX2 after 7 and 14d,meanwhile 3D+Nano group was the highest.Conclusion:Nano-micro composite structure benefits cells to spread and proliferation,accelerates their differentiation toward osteoblasts at the same time. |
