Experimental Study On Cerebral Nerve Function Of Rat After 40 Minutes Of Deep Hypothermic Circulatory Arrest

Objective:The model of deep hypothermic circulatory bypass in rats was established and improved to evaluate the neurological function of rat after 40 minutes deep hypothermic circulatory arrest(DHCA).Methods:Twenty Sprague-Dawley rats were involved and were divided to sham group(n=10)and DHCA group(n=10).Behavioral and cognitive function was tested by Morris maze test and open field test in two groups.Result:The model of deep hypothermic circulatory bypass in rats was established and improved successfully with 90%survival.Morris maze test showed a decline in the memory and learning ability which was proved by longer latency time in the DHCA group.Open field test showed a change of emotion on PD3,which was proved by longer time spent in the center in the DHCA group.Conclusion:Behavioral and cognitive function of rat is affected by 40 minutes deep hypothermic circulatory arrest(DHCA).The model could be used to study for cerebral damage and protection resulting from DHCA.