AMD3100 Attenuates Rats Temporomandibular Joint Osteoarthritis Suppressing Expressions Of MMP-13?IL-1 And P38MAPK

Objective:To investigate the effect of SDF-1 on expression of IL-1??MMP13 and P38MAPK in rat temporomandibular joint osteoarthritis(TMJOA)by est?ablishing a TMJOA Animal model using intra-articular injection of monosodium iodoacetate(MIA).Method:48 Wistar rats were allocated to the control group,the pathologic model group,the high concentration antagonist groups and the low concentration antagonist groups(12 rats per group).TMJOA was induce by injection of monosodium iodoacetate(MIA)into the upper compartment of bilateral TMJs of rats.SDF-1/CXCR4 axis antagonist AMD3100 were injected into the upper compartment of bilateral TMJs of rats in the antagonist groups after the pathologic model was established while the rats in the other groups were injected same amount of normal saline in the same way.Then all rats were sacrificed after 4 weeks.HE and Safranin O-fast green(S.O)staining were performed to evaluate the severity of TMJOA.The expressions of MMP-13?IL-1?were detected by RT-PCR and Immunohistochemistry and the level of p38MAPK were tested by Immunohistochemistry.Result:The pathological scores of control group,high or low antagonist concentration groups and pathologic model group were 0.75±0.43?510.71?8±0.71?11.5±0.5.The expression of p-p38 were(14.3±2.2)%?(41.5±2.2)%?(60.0±3.8)%?(74.9±3.5)%.The expression of IL-1? were(12.8±1.0)%?(40.1±2.3)%?(64.2±4.4)%?(81.2±3.5)%.The expression of IL-1?mRNA were 0.97±0.11?3.38±0.48?5.03±0.23?6.35±0.35.The expression of MMP-13 were(13.22±1.2)%?(38.2±2.3)%?(62.1±3.3)%?(80.9±3.0)%.The expression of MMP-13mRNA were 0.96±0.18?2.54±0.41?5.24±0.45?6.31±0.35.The expression of SDF-1 in control group and pathologic model group were(26.1 ±3.2)%?(75.2±4.5)%.The expression of SDF-lmRNA were 1.02±0.14?6.26±0.45.Results shows with the increasing amount of antagonist applied,the severity of TMJOA and the expressions of MMP-13?IL-1?and p38MAPK were significantly reduced.Conclusions:The pathological effect of SDF-1 on TMJOA is correlated with the increasing expression of MMP-13 and IL-1? and the activation of p38MAPK.With the increasing amount of AMD3100,expressions of MMP-13,IL-1?and p38MAPK decreased in a dose-dependent manner.AMD3100 can block the pathological effect of SDF-1 and attenuate TMJOA.