| ObjectiveProviding evidence for mouse esophageal precancerous xlesion model with establishing KM mouse esophageal precancerous lesion model by chemical carcinogen 4-nitroquinoline oxide-1-(4NQO);And observing the effect of Seabuckthorn dry emulsion on different degrees of esophageal pathological changes with Hematein Eosin and light microscope.MethodsA total of 145 healthy KM mice,male and female,were randomLy divided into 5 groups:group A(normal group)10 mice,group B(simple carcinogenesis group)45 mice,group C(Sea Buckthorn Dry Emulsion treatment group)45 mice,group D(All-Trans-Retinoic Acid treatment group)45 mice.(1)Group A:at 1d?24w of the experiment,given normal diet and drinking distilled water freely,without other intervention;Group B:at Id?14w of the experiment,drinking solution of 4NQO freely,and than changed into distilled water by the end of 14w;Group C:at ld?14w of the experiment,drinking solution of 4NQO freely,and than changed into distilled water by the end of 14w,and Sea Buckthorn Dry Emulsion was added by gastric perfusion until the end of the experiment;Group D:at Id?14w of the experiment,drinking solution of 4NQO freely,than changed into distilled water and All-Trans-Retinoic Acid was added by gastric perfusion until the end of the experiment by the end of 14w;(2)At the end of 10w?12w?14w,2 mice in group A?B were dissected,respectively;Pathological test results confirmed that the esophageal precancerous lesion model successfully established at the end of 14w,At the end of 19w,2 mice in group A were dissected,20 mice in groupB?C?D,respectively.At the end of 24w,dissect all the remaining mice.Observing the 4NQO induced esophageal precancerous change process by HE staining and histopathological detection,meantime,comparing each group of the degree of esophageal precancerous,analyzing the effect of Seabuckthom dry emulsion on mice esophageal precancerous lesions.Results(1)At the end of 14w,5 cases in group B showed 3 cases of mild dysplasia?1 case of moderate dysplasia and 1 case of severe dysplasia,the rate of dysplasia(mild and moderate and severe dysplasia)was 100%(5/5);Since all the cases in this study was randomized,so it can be confirmed that esophageal precancerous lesion model was established in B,C,D group successfully;(2)At the end of 19w:Cancer rate showed no significant difference(Fisher P=0.927)when group A?B?C?D were compared over the same period;Light and moderate dysplasia,severe dysplasia and canceration rates showed no significant difference(?~2=2.679,P>0.05);(3)At the end of 24w:Cancer rate of group C was significantly lower than that of group B(?~2=6.561,P<0.05)when group B?C were compared over the same period;Cancer rate of group D was significantly lower than that of group B(?~2=10.506,P<0.05)when group B?D were compared over the same period;But when group C compared with group D at the same period,the cancer rate showed no significant difference(?~2=0.739,P>0.05).Conclusion(1)The KM mouse model of esophageal precancerous lesion can be successfully established by drinking 4NQO solution of 0.1g/mL concentration for 14 weeks;(2)Seabuckthorn Dry Emulsion has a certain effect on the esophageal precancerous lesions,which can slow down the progression of esophageal precancerous;And the effect was not worse than All-Trans-Retinoic Acid.ObjectiveTo explore the expression change of ANO1 and EGFR protein in esophageal epithelial dysplasia and cancerous process,and the possibility of them to be new biological markers of early events for esophageal carcinogenesis.MethodsEstablish mice esophageal epithelial dysplasia model with 4NQO,contrast esophagus histopathologic changes observed with Hematein Eosin and light microscope in different periods of mice,detect different esophageal lesions in mice tissues of EGFR and ANO1 protein expression level,compare the expression changes of ANO1 and EGFR in esophagus normal tissues,dysplasia and cancerous tissues by immunohistochemistry.Results(1)The expression of ANO1 were in cytoplasm,the expression of ANO1 protein in the various lesions of esophagus,including normal?mild dysplasia?moderate dysplasia?severe dysplasia and cancerous tissues in ANOl protein positive expression rates were 0%(0/14)?0%(0/25)?2.56%(1/39)?34.78%(8/23)and 50.00%(22/44),respectively.The positive rate of ANO1 in cancer tissues was significantly higher than that in normal tissues(x2=11.278,P<0.05),mild dysplasia(?~2=18.351,P<0.05)and moderate dysplasia(x2=23.224,P<0.05);the positive rate of ANO1 in severe dysplasia tissues was significantly higher than that in normal tissue(fisher P=0.015)?mild dysplasia(x2=8.081,P<0.05)and moderate dysplasia(?~2=9.645,p<0.05);(2)The expression of EGFR were in cytoplasm,the expression of EGFR protein in the various lesions of esophagus,including normal?mild dysplasia?moderate dysplasia?severe dysplasia and cancerous tissues in EGFR protein positive expression rates were 7.14%(1/14),16.00%(4/25),23.08%(9/39),52.17%(12/23)and 59.09%(26/44),respectively.The positive rate of EGFR in cancer tissues was significantly higher than that in normal tissues(?~2=11.519,P<0.05),mild dysplasia(X2=12.046,P<0.05)and moderate dysplasia(x2=10.996,P<0.05);the positive rate of EGFR in severe dysplasia tissues was significantly higher than that in normal tissue(fisher P=0.011),mild dysplasia(x2=7.054,P<0.05)and moderate dysplasia(?~2=5.469,P<0.05).(3)67 cases of cancerous tissues,AN01 positive group in the positive rate of EGFR 93.33%(28/30)when EGFR negative rate in ANO1 negative group was 72.97%(27/37),expression correlation coefficient of ANO1 and EGFR r=0.665,and the correlation was significant(P<0.05).Conclusion(1)The expression of ANO1 showed high specificity in severe dysplasia and carcinoma tissues,suggesting that they are involved in the development of esophageal carcinoma,and may become a target of biological markers of esophageal cancer and new medications(2)The expression of ANO1 and EGFR has a significant positive correlation in esophageal-dysplasia and cancerous tissue,the complex two-way interaction between the them may be the mechanism. |
PDF Full Text Request