Expression Of MiRNA21?miRNA29b And MiRNA133a In Myocardial Tissue And Circulation Of Rats With Myocardial Fibrosis

In this experiment Myocardial fibrosis model was established in rats,irbesartan and simvastatin were used to intervened,and investigating the regulation expression of miRNA21,miRNA29 b and miRNA133 a in myocardial fibrosis and whether the content of serum is consistent with the expression of tissue.We chose 50 male SD rats were randomly divided into 5 groups,two weeks after abdominal aortic coarctation,Intragastric administration was given to sham group and model group with 0.5% sodium carboxymethyl cellulose(0.5ml/d),irbesartan group(irbesartan 20 mg.Kg/d),simvastatin group(simvastatin 5mg.Kg/d)and combination group(irbesartan 20 mg.Kg/d+ 5mg.Kg/d simvastatin)for 8 weeks.Measure body weight,blood pressure,heart index,serum Ang II and CVF,LVD,LVS,IVS diameter measured by ultrasound,pathological staining with HE and Masson.Immunohistochemistry and Western blot are used to determine TGF-?1,CTGF and ATR1 protein content,qPCR method was used to detect the expression levels of miRNA21,miRNA29 b and miRNA133 a in tissue and serum in rats of each group.Through experiments,we know that compared with the sham group,the heart index,IVS,CVF,TGF?1 and CTGF increased in model group,and the body weight and ATR1 decreased(P<0.05);Compared with the model group,body weight gain in irbesartan and simvastatin group,loss in combination group,cardiac index,EF,CVF,TGF-beta 1,CTGF and ATR1 decreased in irbesartan and simvastatin group(P<0.05),and except ATR1 increased in combination group(P<0.05),the rest were lower than the irbesartan and simvastatin group(P<0.05).miRNA21 in Ir group and Model group decreased,increased in Si group and Ir+Si group;miRNA29b increased in Si group,decreased in Ir group,Ir+Si group and Model group;miRNA133a decreased in Ir group,Si group,Ir+Si group and Model group.From this point of view,Irbesartan and simvastatin could alleviate myocardial fibrosis,and the combination of 2drugs is more effective,although the ATR1 increased with unkonw reason.Pay attention to choise according to indications to reduce adverse drug reactions.The expression of miRNA21,miRNA29 b,miRNA133a in the serum of rats with fibrosis are abnormal compared with the Sham group.