Preparation And Characterization Of Hollow Bioactive Glass Composite Materials For Bone Repair

In recent years,due to the aging population,frequent traffic accidents and the increase of patients with various incurable diseases,resulting in bone tissue defects and organ damage phenomenon is also increasing.Preparing and searching ideal biological substitute materials for bone tissue repairing has become a hot spot in bone tissue engineering,and the biomedical textiles for bone tissue repairing has also become an important research direction of modern biomedical textiles.Silk fibroin is a kind of polymer fibrin which is degummed by natural silk.Because of its unique amino acid,silk fibroin possesses good biocompatibility and biodegradability,which make silk fibroin become the focus of researchers on biomedical field especially bone repair material.However,the bone bonding ability of silk fibroin is poor,and biological activity is low.These disadvantages limit its application and development in bone repair materials.In this paper,SF/PEO/HBG composite fiber membranes were prepared by the composite silk fibroin and hollow bio-glass spheres,and the addition of polyethylene oxide improve the elasticity of silk fibroin.Taking this composite as research object,the properties of this composite including HBG and SF/PEO/HBG composite fiber membranes were prepared,bioactivity and investigated.The obtained results were described as follows:(1)Preparation and characterization of the hollow bioactive glass nanoparticle.PAA @ BG core-shell bio-glass spheres were prepared by the improved sol-gel method using poly acrylic acid(PAA)as the soft template.After removal of PAA by calcining,hollow bio-glass nanoparticle(HBG)was obtained.The morphologies,structures,sizes of these as-prepared HBG were characterized by different techniques,such as FE-SEM,TEM,FTIR,EDS and TG.The effect of preparation time on hollow bio-glass spheres was studied.The preferred reaction time was 72 hours.The calcium salt and phosphorus could be bonded on the hollow SiO2 surface.Finally,hollow bio-glass spheres with good morphology,structures and properties were prepared.The TG showed that when the calcination temperature was 550?,the PAA and unreacted calcium and phosphorus in the sample were completely decomposed.(2)Preparation and characterization of the hollow bioactive glass nanoparticle withdifferent particle diameters.By changing the mass ratio of TEOS/PAA,the size of hollow bioactive glass nanoparticle was controlled.The structure,morphology and properties of the hollow bioactive glass nanoparticle were characterized by FE-SEM,TEM,particle size distribution,FT-IR,EDS and XRD.The bioactivity was investigated by in vitro.We found that HBG had spherical shapes?smooth surfaces without any coagulation and also had obvious hollow structures,and had good bioactivity.(3)Preparation and characterization of the SF/PEO/HBG Composite materials for bone repair.Silk fibroin solution was prepared by degumming and dissolving the silk.After adding PEO and stirring,the mixed solution of SF/PEO was prepared.SF/PEO/HBG Composite materials were prepared by electrospinning.The morphology and properties of the SF/PEO/HBG bone repair composite were characterized by FE-SEM,FT-IR,EDS and XRD.The bioactivity was investigated by in vitro.We found that the SF/PEO/HBG composite materials had fiber morphology,smooth surface,network structure and bioactivity.But the fiber thickness is not uniform.In summary,SF/PEO/HBG composite materials possessed excellent bioactivity and properties.All these findings indicate that SF/PEO/HBG composite materials are potential bioactive materials for bone repair and treatment.