The Relationship Of MiR-21?miR-375 Expression And Clinicopathological Factor In Pancreatic Ductal Adenocarcinoma

Object:To investigate the expression of microRNA-21(miR-21)?microRNA-375(miR-375)in pancreatic ductal adenocarcinoma(PDAC)and corresponding normal pancreatic epithelial tissues,detect its relationship with clinicopathological features.Analysis expression of Spry2?AEG-1 in the PDAC cancer and corresponding normal pancreatic ductal adenocarcinoma epithelial tissues.The relationship between the expression of miR-21 or Spry2 and clinical pathologic parameters in the PDAC was analyzed.The relationship between the expression of miR375 or AEG-1 and clinical pathologic parameters in the PDAC was also analyzed.Methods:The RT-qPCR method was used to assess the abundance of miR-21?miR-375 in 50 primary the PDAC specimens which were diagnosed and operated in the second affiliated Hospital of Zhejiang university between 2012 and 2014,in comparison with the corresponding adjacent non-tumor tissues.The relationship between the expression of miRNAs and clinicopathological features was analyzed Immunohistochemical staining to detect expression of Spry2?AEG-1 protein in the PDAC and corresponding pancreatic epithelial tissues,The relationship between the expression of miR-21 or Spry2 and clinical pathologic parameters in the PDAC was analyzed.The relationship between the expression of miR-375 or AEG-1 and clinical pathologic parameters in the PDAC was also analyzed.Results:1.The expression of miR-21 was significantly elevated in the PDAC compared with their non-tumor counterparts from patients undergoing pancreatic resection.The expression of miR-375 was significantly decreased in the PDAC compared with their non-tumor counterparts from patients undergoing pancreatic resection.2.No significant relationship was found between the expression of miR-21/miR-375 and clinic-pathological features.3.There was negative relationship between miR-21 and Spry2 mRNA in the PDAC(r=-0.386,p=0.033).There was negative relationship between miR-155and AEG-1 mRNA in the PDAC(r=-0.370,p=0.045).4.Expression of Spry2 protein is significantly higher in non-tumor counterparts epithelial tissues than in the PDAC tissues.Expression of AEG-1 protein is significantly lower in non-tumor counterparts epithelial tissues than in the PD AC tissues.5.Expression of Spry2 mRNA related with lymph node metastasis in the PD AC.Expression of Spry2 protein related with lymph node metastasis in the PD AC.Expression of AEG-I protein related with lymph node metastasis?tumor size in the PD AC.There was no relationship between.Expression of AEG-1 mRNA and clinicopathological factor in the PD AC.6.There was negative relationship between miR-21 and Spry2 proteins in the PDAC(r=-0.398,p=0.030,P<0.05).There was no relationship between miR-375 and AEG-1 proteins in the PDAC(r=-0.313,p=0.128 P>0.05).Conclusion:miR-21 and miR-375 expression were significantly elevated in the PDCA,it is possible that miR-21 and miR375 play significant roles in the PDAC through the control of Spry2 and AEG-1.