Effects Of Targeting For RAD18 On The Proliferation And Chemosensitivity Of Human Esophageal Squamous Cell Carcinoma

Objective:To investigate effects of targeting for RAD 18 on the proliferation and chemosensitivity of human esophageal squamous cell carcinoma.Methods:Real-time PCR was used to detect the expression of RAD 18 and CyclinDl in esophageal carcinoma tissues.ECA-109,human esophageal squamous cell lines,was chosen as a model,and RAD18-siRNA was transfected into this model using Lipofectamine3000 method.Western blotting method was performed to measure RAD 18 and CyclinD1 expression.CCK-8 assay was used to examine cell proliferation and chemotherapy drug sensitivity.Flow cytometry was used to determine cell cycle.The correlation between RAD 18 and CyclinDl gene expression was analyzed by Pearson's test in esophageal squamous cell carcinoma.Result:The mRNA expression of RAD18 in esophageal squamous cell carcinoma tissues was significantly higher than that in para-cancerous tissues.In Eca-109 cell lines,downregulation of RAD 18 expression led to a decrease in cell proliferation,and an increase in cellular sensitivity to cisplatin and 5-fluorouracil.Its mechanism was partly attributed to suppression of cyclin D1 expression and G1 phase arrest.In esophageal squamous cell carcinoma tissues,there was an obvious positive correlation between the expression of RAD18 and CyclinDl mRNA(r = 0.478,P = 0.001).Conclusion:RAD18 plays an important role in ESCC progression and chemoresistance,and is a potential diagnostic marker and therapeutic target for ESCC.