Characteristic Variation Research On Cancer Genome

Currently,cancer is one of the diseases seriously endanger human health.A large number of studies have indicated that the occurrence and development of cancer often involve multiple genetic abnormalities,by detecting abnormalities in these cancer-related genes,it may prompt the imechanism of cancer and as some clinical diagnostic criteria.Copy number variation(CNV)is an inportant part of the genome structural variation,the its site mutation rate is far higher than the single nucleotide polymorphism(SNP),and the range of coverage in the whole genome is very large.CNV can reveal the internal complex relationship between genes and human diseases,so the are more significant effects on human health and diseases.Array-based comparative genomic hybridization(aCGH)combines the advantages of comparative genomic hybridization and DNA chip technology,it is high throughput,rapid and easy automation,the data is easyly to be analysised etc.It provided the most reliable and robust method on searching and discovering new CNVs since it has been applied to genome-wide scan.In this study,the high qualitative and resolution probes covering the whole genome of the human genome have been prepared with a series of polymerase chain reaction(PCR),which laid the foundation for the preparation of whole-genome aCGH chips.In order to reduce cost,a high yield,convenient Bst DNA polymerase large fragment expression system has been designed to gain a lot of high activity of Bst DNA polymerase large fragjment.Cancer-specific aCGH arrays contain 4850 probe bins have been prepared,and its feasibiliy and accuracy have been indicated.The whole genome DNA in formalin-fixed paraffin-embedded(FFPE)tissues have been extracted.The method that enable whole genome amplification(WGA)using DNA extracted from FFPE tissues with Bst DNA polymerase large fragment has been explored.aCGH study in cancer CNVs have got a lot of hybridization results and datas of gastric cancer samples.It provided some important research methods and effective means for the detection of cancers.It is possible for general laboratory to widely use high-throughput aCGH technique.aCGH can find multiple genes of CNVs,so it is better to be applied in clinical diagnosis.