| BackgroundObesity is associated with an overexpansion of adipose tissue,along with hyperlipidemia,inflammation,and thrombosis.Research to develop nutritional interventions to prevent or treat obesity and its associated diseases is greatly needed.Yeast polysaccharide(YPS)is used as adjuncts in food to provide a wide variety of health benefits.YPS has attracted attention as potential cholesterol lowering additives by entrapping bile acids or reducing motility in the upper part of the intestinal tract,increased fiber digestion,increased fiber digestion.Aim of studythis paper seek to explores the role of YPS in the metabolism of lipids,the effects in biochemistry analysis,antioxidant activity,pathological lesion and gene expression in mice fed with HFD for seven weeks.The experiment was conducted to investigate the effects of yeast polysaccharide on mice lipid metabolism,pathological lesions,antioxidant activity,biochemical index and genetic expression induced by feeding a high fat diet and to explore YPS mechanism.MethodSixty female ICR mice at age of 4 weeks(body weights around 23 g)were purchased from the Center of Laboratory Animals,Nanjing Medical University(Nanjing,Jiangsu,China).they were divided into 4 groups by a randomized complete block design containing woodchip bedding(15 per group).Mice in Group A were fed a basal diet and served as normal diet(ND).Mice in group B fed a high fat diet(HFD),Mice in group C were fed the HFD supplemented with 250 mg/kg body weight YPS orally administered three times a week(HFD+YPS 250)group.Mice in group D were fed a high fat diet supplemented with 450 mg/kg body weight YPS orally administered three times a week(HFD+YPS 450).They were acclimatized at a normal standard temperature of 20-24? and a relative humidity of 50-70%with a 12 h light-dark cycle for 1 week on mice's chow diet only.The mice were given these diets for seven weeks along with water ad libitum.Weekly measurements of weight were recorded.All procedures using animals obtained the approval of the Animal Experiment Committee of China,and conducted in accordance with the Guide for the Care and Use of Laboratory Animals at Nanjing Animal Hospital.ResultsConsumption of an HFD resulted in increased body weight,serum lipids,relative adipose tissues size,and liver fat accumulation.However,administration of YPSsignificantly decreased body weight gain,food intake,food efficiency ratio,hepatic cholesterol level,and adipose tissue size in a dose-dependent manner as well as occurrence of fatty liver deposits and steatosis.In addition,YPS also attenuated lipid hydro peroxide levels by increasing the enzymatic antioxidants(SOD activity and GSH,MDA levels)in the mice.To elucidate the mechanism of action of YPS,beta-oxidation genes,lipogenesis genes and cholesterol mechanism related gene were measured in liver.mRNA expression results showed inhibition of lipogenesis via down-regulation of SREBP1,FAS and SCD1 mRNA levels and increased beta-oxidation via up-regulation of PPAR? and CPT2 mRNA levels.Therefore,these results suggest that dietary supplementation with YPS may be useful to ameliorate high fat induced obesity through fat digestion inhibition.ConclusionIn conclusion,the present study demonstrated that YPS treatment exerts anti-obesity action through b-glucan contents beneficial,improvement of the antioxidant activities within hepatic cells,lowering triglycerides and the enhancement of the lipid metabolism in liver due to the increasing of serum HDL-cholesterol and the decreasing of LDL-cholesterol modulating the gene expression related to lipid metabolism via down-regulation of SREBP1,FAS and SCD1 mRNA levels and increased ?-oxidation via up-regulation of PPARa and CPT2 mRNA levels.High dose exert higher significant difference. |
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