| Objective:This dissertation attempts to observe the effect of Fuzi Lizhong decoction on expression of colonic mucosal nuclear factor-Kb (NF-? B) and serum tumor necrosis interleukin-8 (IL-8) of the spleen and kidney Yang deficiency type ulcerative colitis(UC) rats.Consequently, it will do favor to supply experimental evidence to treat UC and the wider clinical application of Fuzi Lizhong decoction.Methods:?Modeling:On the basis of previous studies on the preparation of ulcerative colitis (spleen kidney Yang deficiency type) model in rats.?Treatment:42 male rats with UC(caused by Yang deficiency of spleen and kidney) are scattered into 5 groups stochastically, that is to say,10 rats belong to the model control group, and 8 rats consist of the western medicine control group.Meanwhile, groups of high-dose, medial-dose and low-dose of Fuzi Lizhong decoction have 8 rats respectively. On the other hand, there are also 8 rats regarded as normal control group.Rats in the normal control group and model control one are given enema by 3ml normal saline per day, and rats in the western medicine control group are given enema by 3ml mixed suspension of willow nitrogen sulfonyl pyridine enteric-coated metformin hydrochloride (35mg/ml), once a day. As for the other 3 groups, rats in the high-dose are offered enema by 3ml mixed suspension of high concentration (1.18g/ml) of Fuzi Lizhong decoction per day, and rats in the medial-dose are also provided clysis by 3ml mixed suspension of high concentration (0.59g/ml) of Fuzi Lizhong decoction per 24h, additionally, rats in the low-dose are supplied stomach lavage by 3ml mixed suspension of low concentration (0.295g/ml) of Fuzi Lizhong decoction per 24h.During the experiment, daily observation is about feaces, food intake, morphology, fur and movement etc, and it needs measurement of weight and anal temperature once three days.With 14 days of continuous infusion, then rats are just supplied by water (no food) in 24 hours, after that, each rat anesthetized with 3ml urethane(20%) is taken blood (5ml) from ventral aorta.Then put the blood in centrifuge with 4? 3000r/min to separate the serum, after 10mins, the centrifugation ends and put them in the refrigerator with sub-zero 20?.It's expected that ELISA would be employed to check the contents of IL-8 in serum of rats.Subsequently execute the rats by cervical dislocation and open the abdomen, and take out colon which is 4 to 10 centimeters above from the anal.Then shear and clean up the tissues, and fix the specimens by neutral formalin (10%) to make pathological sections and observe them through light microscope.Observations are divided into two parts, one part with 10% neutral formalin fixed specimens, making the pathological slice, then observe each group rats colon tissue, another part was kept in a deposit-80 ?, using RT-PCR to detect the NF-? B content in colon tissue.Results::? After treatment with retention enema, aconite each dose group of rats eating soup, stool, weight, shape, colour, activities and anus temperature, etc are better than that of the model control group.?According to observation by naked eyes and light microscope, situation of inflammation, edema and hyperemia of the colon tissue infused by Fuzi Lizhong decoction is better than that of the model control group.?Compared with normal group, Fuzi Lizhong decoction significantly improve the level of mucosal inflammation and damage score.Compared with model group, Fuzi Lizhong Tang significantly improve the level of mucosal inflammation and damage score and reduce the expression of colonic mucosal NF-? B and serum IL-8of the spleen and kidney Yang deficiency type UC rats (P<0.05)Conclusion:?Fuzi Lizhong decoction is able to improve the spleen and kidney Yang deficiency type UC rats eating less, thin pond and mucus is purulent blood stool, weight loss, unresponsive, limbs don't accept, it withered and activity to reduce symptoms such as color, can reduce the pathological damage and inflammation model in rats.?Fuzi Lizhong decoction has anti-inflammatory and repair function on spleen and kidney Yang deficiency type UC ratThe possible mechanism is inhibition of UC rat intestinal mucosa NF-?B activation, thus inhibiting the expression of IL-8 and reducing inflammatory exudation. |
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