Study On The Effect Of Modified Renshen Wumei Decoction On The Structure Of Intestinal Flora Of Diarrhea Rats

Objective:It is to study the influence of compound Modified Renshen Wumei Decoction on the Characteristics of the diarrhea model rats intestinal flora by polymerase chain reaction-denaturing gradient gel electrophoresis technology.Methods:Randomly selected 12 from 48 SD rats for the control group, and the remaining 36 according to the literature method were made into diarrhea model by combined factors. After the success of the building were randomly divided into model group, western medicine, Chinese medicine group, each group of 12. Building 21d to drug intervention, the control group and model group to saline, the rest group were given mammy love,Modified Renshen Wumei Decoction,the average of 1/d, continuous administration 7d.(1) Record the date about body weight,the number of loose stools and loose stools rate of the diarrhea rats'.Get the colon tissue of rats and observe the change of pathological of rats in each groups.(2) Collect all rats feces in each group,extract fecal bacteria genomic DNA, PCR amplified bacteria 16S rDNA gene V3 variable region. DGGE method for detecting PCR products. Advantages bands were recovered for sequencing and identification of bacteria species.Results:(1)Compared with the rats of model group,the rats of Chinese medicine group grow obviously on body weight (p<0.01).Compared with the rats of model group,the rats'had a good cure on the symptom of diarrhea in Chinese medicine group (p<0.01). Compared with the rats of model group,the rats of Chinese medicine group has a good recover on the mucosa pathology (p<0.05).There's no differences between rats of the rest group,the rats of Chinese medicine group on the mucosa pathology (p>0.05).(2)drug treatment of diarrhea model rats after the highest relative abundance of intestinal microbial was Firmicutes bacteria categories, followed by Bacteroidetes and Proteobacteria categories. Modified Renshen Wumei Decoction in rats after treatment intestinal Firmicutes bacteria contentincreased significantly (P<0.01), while the intestinal bacteroidetes bacteria content decreased significantly (P<0.01), Proteobacteria categories showed no significant change (P>0.05). Compared with the western medicine group, after Modified Renshen Wumei Decoction rat microbiota much closer to the control group.Conclusions:(1)Modified Modified Renshen Wumei Decoction can cure the diarrhea rats and improve the mucosal pathology well.(2)Modified Modified Renshen Wumei Decoction can improve the gut microflora environment.Compared with western medicine Modified Renshen Wumei decoction can take beneficial action on the intestinal flora balance.Modified Renshen Wumei decoction may influence the structure of the intestinal flora to correct flora disturbance to cure the diarrhea rats.