The Effects Of Corn Oil And Compound Oil Of Corn Oil And Lard On Lipid Metabolism And Liver And Kidney Functions In Mice

Objective:To investigate the effects of corn oil and compound oil of corn oil and lard on lipid metabolism and liver and kidney functions in mice, to provide reference frames and a new kind choice for people to eat healthy oil.Methods:Eight-week old male 40 SPF C57BL/6J male mice whose weighing about 24g were randomly divided into 4 groups, following are the four different groups:Group A1,3.8% compound oil group (57%corn oil:43% lard):Group A2.6.5% compound oil group (57%corn oil:43% lard); Group B1,3.8% corn oil:Group B2.6.5% corn oil with ten of each group. On the basis of feeding the basal diet, every group was fed by adding 3.8% and 6.5% of corn oil and compound oil of corn oil and lard for 12 weeks, then weighing, blood was collected, body fat weight and liver weight were taken for calculating liver index and body fat rate. Later blood lipids. blood glucose, serum protein, liver lipids level and liver function, renal function as well as oxidative damage index were detected.Results:(1)The weight and body fat rate of 6.5% compound oil group and 6.5% corn oil group are very significantly higher than those in the same oil group(P<0.01), the liver index of the 3.8% compound oil group and the 6.5% corn oil group is very significantly lower than that of the 3.8% of corn oil(P<0.01). (2) By the comparison of compound oil groups, there is no obvious difference among TC. TG. HDL-C and LDL-C (P>0.05). the TC, TG and LDL-C of 6.5% corn oil group is extremely very significantly higher than 3.8% corn oil group (P<0.01). and the difference of HDL-C is not significant between 6.5% corn oil group and 3.8% corn oil group (P>0.05). TC. TG of 3.8% compound oil group are very higher than that of the 3.8% corn oil group (P<0.01), HDL-C of 3.8% compound oil group is significantly higher than that of the 3.8% corn oil group (.P<0.05). HDL-C of 6.5%corn oil group is significantly lower than that of the 6.5% compound oil group (P<0.05). There is no significant difference in blood glucose (P>0.05). (3) There is no significant difference of ALT in each group (P<0.05). The comparison of compound oil groups, there is no obvious difference among AST. ALP. LDH (P>0.05). AST. ALP. LDH is no obvious difference between the 3.8% compound oil group and 3.8% compound oil group (P>0.05). AST, ALP, LDH of 6.5% compound oil group is very lower than 6.5%corn oil group (P<0.01). ALP, LDH of 6.5% corn oil group are significantly higher than the same oil of low level group (P<0.01), it's AST is very significantly higher than that of the same oil of low level. (4)There is no significant difference of TP, G, ALB between the compound oil groups (P>0.05), but TP, G of 3.8%compound oil group are significantly higher than that of the 6.5%corn oil group (P<0.05). (5) Liver lipid levels of TC, TG are not significantly different in each group (P<0.05), the level of MDA in the compound oil groups is significantly lower than that in the same level corn oil groups (P<0.05), the difference is not significant between the same level oil groups (,P>0.05). T-AOC, SOD and GSH-PX of 3.8% compound oil group are significantly higher than 6.5% compound oil group (P<0.05). However, T-AOC, SOD and GSH-PX levels are not significantly different between the two groups which are at the same level of oil. (6) There is no obvious difference among UA, UREA in each group (P>0.05). CREA is no obvious difference in compound oil groups. CREA of 3.8% corn oil group is significantly lower than that of the 6.5%corn oil group (P<0.05). CREA of 6.5% compound oil group is very significantly lower than that of the 6.5% corn oil group (P<0.01).Conclusion:(1) The study finds that 6.5%level oil has obvious influence in significantly increasing in body weight and body fat rate, elevating blood lipids, reducing liver function and kidney function compared with the long term intake 3.8% level of oil, which is harmful to health,3.8% level of intake oil is more beneficial to body health. So, of decreasing the oil intake should be suggested. (2) The study finds that 3.8% compound oil and 3.8% corn oil with the long term intake have the same effects on body weight, liver index, body fat rate, blood lipid. blood glucose. But 3.8% compound oil with long term intake can significantly reduce the content of MDA and CREA level and significantly increase the content of TP and G level on body as well as it is more beneficial to liver function, kidney function and enhances immunity. In daily life, intake of 3.8% level compound oil is better compared with the same level com oil. (3) The study finds that 6.5% compound oil compared with 6.5% com oil with the long term intake has almost same effects in the body weight, liver index, blood glucose and total antioxidant capacity. But compared with the same level of com oil, the 6.5% level of the compound oil can significantly increase the level of HDL-C, significantly decrease the level of UREA, MDA and very significantly decrease the level of CREA, TC. It shows that the effects of high level compound oil with long-term intake are better than single corn oil for health.