Myroilson Decullarized Nerve Scaffold Promotes Neural Differentiation Of DPSCs

Objective:To characterize the property of a novel nerve scaffold on proliferation of seeding cells which decullarized by treatment of hydrolyse Myroilysin,and to test the application of this scaffold in promoting seeded DPSC differentiaite into neuron.Methods:Scaffolds were prepared with rat sciatic nerves by either myroilysin hydrolysis or traditional Sondell chemical way.Efection to hDPSC of these two kindsof scaffolds and the vestigital during their preparation was investigated by cck-8 after cultured with DPSC.Capacity of absorbing seeding cells and promoting their differentiation were evaluated by immunofluorescence and western blotting.Results:1.The isolated human dental pulp stem cells had high proliferative activity after separation,purification and amplification.The stem cell marker,STRO-1,was positive and the neurotransmitter protein ?-? tubulin was positive.2.Myroilysin have less chance to hurt seeding cells or affect their proliferation than traditional chemical materials.When cultured in the lixivium derived from scaffolds,proliferation of DPSC didn't reveal statistical differences,indicating the scaffold would be no toxic to the seeding cells.3.Immunofluorescence and western blotting showed the myroilysin prepared scaffold significantly promoted the neural differentiation of DPSC indicated by expression of NeuN.Conclusion:The acellular nerve scaffold via Myroilysin hydrolysis provides better three-dimensional situation than traditional chemical scaffold without mechanical property loss.Reserving more natural structure,it promote localization and neural-like function of human dental pulp stem cell(hDPSC).Fine biocompatibility was shown and no-cytotoxicity was noticed.These perfect property made it possible to load seeding cells in tissue engineering of peripheral nerve injury treatment.