The Effect Of Pterocarya Stenoptera On Proliferation And Apoptosis Of Rheumatoid Arthritic Fibroblast-like Synoviocytes

Objective:To observe effect of Pterocarya stenoptera on production of proinflammatory cytokines by Rheumatoid arthritic fibroblast-like synoviocytes(RA-FLS)and on inducing apoptosis of RA-FLS.Methods:Pterocarya stenoptera extract(PSE)was prepare from Pterocarya stenoptera bark by alcohol-chloroform method.Then cultured RA-FLS were treated with different doses of PSE with different time.The influence of PSE on apoptosis of RA-FLS was detected by using flow cytometry,while on inhibition of RA-FLS proliferation was measured by CCK-8 assay.Caspase-3 and caspase-8 expression in PSE-treated RA-FLS were detected by using Western Blotting.IL-8 level in the supernatant of PSE-treated RA-FLS was detected by ELISA.Results:Proliferation of RA-FLS was inhibited by PSE with dose and time-dependent phenomena(P<0.05).Combared with controls,apoptosis of RA-FLS was induced by PSE with different concentration(P<0.01).Apoptotic RA-FLS treated with 2mg/ml concentration were 19.59%,apoptotic cell increased to 42.03%when treated with 2.5mg/ml concentration of PSE.And coculutred with concentration 3.33mg/ml,apoptotic cell 'increased to 78.42%.The expression of caspase-3 and caspase-8 protein in RA-FLS was down-regulated by PSE(P<0.05).And the IL-8 levels was significantly decreased in supernatant of PSE-teated RA-FLS with concentration 1.25mg/ml of time dependence(P<0.05).Conclusion:PSE might have potential therapeutic efficacy to RA by inducing apoptosis of RA-FLS and by downreguating production of IL-8.