Effect Of Gypenosides On RAGE And TGF-?1 Expression In Human Mesangial Cells Induced By AGEs

Objective: To observe the effect of Gypenosides(GP)on the expression of receptor for advanced glycated endproducts(RAGE)and transforming growth factor-?1(TGF-?1)in human mesangial cells(HMCs)induced by AGEs.Methods: HMCs were cultured in DMEM of low glucose containing 15% fetal bovine serum in vitro,which were divided into four groups: the normal group?model group?GP group?positive control group.In addition to the normal group,According to the MTT results of the previous experiment the remaining three groups was stimulated by AGEs(200mg·L-1).Furthermore,GP group was respectively intervened different concentrations from the low to high(25?75?175 mg·L-1)of GP,while the positive control group was given(0.1mmol·L-1)of aminoguanidine hydrochloride.The expression of RAGE and TGF-?1 protein of each group was detected by Western blotting technology;the expression of RAGE and TGF-?1 mRNA of each group was detected by RT-PCR technology.Results: Cells in vitro culture environment,Compared with the normal group,which of induced by AGEs in HMCs,the high expression of RAGE?TGF-?1 protein and mRNA,the difference was significant(P < 0.05);compared with the positive control group,GP could obviously reduce the expression of RAGE?TGF-?1 protein in a dose-dependent manners,the difference was significant(P < 0.05).Conclusions:GP can reduce the high expression of RAGE in HMCs induced by AGEs,block AGEs-RAGE this signal pathway and decrease the downstream factor expression of TGF-?1,therefore,which play the role of resistance to DN rennal fibrosis,The early prevention and treatment of DN can play a certain positive significance.