Fabrication And Charaterization Of Electrospun Artificial Dura Mater Scaffold With Micro/Nano-fiberous Structure

In surgical operation,The dura mater is often damaged and needs to be repaired with artifical dural substitutes.The current artificial dural substitutes have many problems,such as disease transmission from biological source,inflammation caused by the difficult degradation of the synthetic materials,and cerebrospinal fluid leakage caused by bad mechanical properties of the natural materials.The ideal dural substitute is still a clinical problem to be solved.In this study,a novel electrospinning strategy was proposed to fabricate a unique nanofibrous dural substitute with layered structure.We did reserach on the dura mater's physical properties and biocompatibility,which builds the foundation of clinical application.The electrospinning process was studied to fabricate aligned nanofiberous membranes,based on which a novel strategy was proposed to manufacturing a layered structure of the artificial dura mater.The results showed that the orientation and mechanical properties of nanofibers were improved with the increase of collector rotating speed.It proposed a method of fabricating a layered structural membrane by alternating electrospinning aligned fiber and random fiber.The thickness of the membranes,micronanoelectronic layered structure,mechanical properties and water absorption were evaluated.The thickness of the dura mater scaffold was about 200~300?m,which was close to that of the natural dura mater.It confirmed the micro/nano-fiberous three-dimensional structure and fiber orientation of the scaffolds by observing the stretching cross-sectional microstructure.Among these,the scaffolds of high and low speed alternately electrospinning 30 min had the best mechanical property with the ultimate tensile of 6.467±1.194 N in parallel and 2.506±0.682 N in vertical,the ultimate stress of 6.116±1.094 MPa in parallel and 3.088±0.735 MPa in vertical and the breaking elongation of 93.45±8.981% in parallel and 171.4±19.363% in vertical.Nanofibrous dura mater was tested in vitro for cell attachment and alignment using3T3 fibroblasts.It revealed that the number of cells aligned fibrous layer was more than random fibrous layer,and the growth properties of the cells had a certain alignement,while random alignement was shown on the random fibrous layer.In the MTT assay,these results indicated that the aligned fibers scaffolds ware non-toxic and could enhance cell proliferation.A significant increase in the cell number on the aligned fibrous layer surface after 5 days of culture was further demonstrated.