Effects Of MiRNA-338 On Model Of Rat With Experimental Autoimmune Neuritis

Objective: To observe the effects of miRNA-338 on model of rat with experimental autoimmune neuritis.Methods: Thirty healthy,pure,female Lewis rats were randomly divided into nomal control group(n=10),miRNA-338 treatment group(n=10)and saline treatment group(n=10).The P0180-199 peptides respectively were injected into plantar subcutaneous of double hind limbs of Lewis rats of miRNA-338 treatment group and saline treatment group to inducing EAN model.Seventh days of immunization,the lentiviral vectors of carrying miRNA-338 were injected into the three indentical injection sites from the shape area of bilateral sciatic nerve of rats of miRNA-338 treatment group,the rats of saline treatment group were injected with the same dose of saline at the same position.From the immunized start recording behavior changes and scores,compared with the peak of the highest score,eighteenth days after the immuization,the treatment effect of rats in each group was evaluated by nerve-muscle action potential,gastrocnemius muscle HE staining,and motor endplate acetylcholinesterase,and toluidine bluesciatic,nerve electron microscopy,and immunohistochemistry,and other indicators of detection.Results:(1)Compared with normal group,miRNA-338 treatment group behavioral changes close to normal,toes grip strength and ability to walk closer to normal,the maximum average score of the peak comparison with the saline treatment group improved significantly(p<0.05);(2)Compared with the saline treatment group,miRNA-338 treatment group electrophysiological testing showed that the peak of the scitic nerve compound action potential conduction velocity VmicroRNA treatment group>Vsalinetreatment group(p<0.05),the amplitude AmicroRNA treatment group>Asaline treatment group(p<0.01);(3)The model animals arised that the the sciatic nerve fibers diameter becomes small,loosely arranged,cross-sectional shape are not uniform,gastrocnemius fibers thinner,lightly stained cytoplasm and other inflammatory degenerative changes,as well as the number of motor endplates acetylcholinesterase reduction etc.Compared with saline treatment group,the above index in miRNA-338 group were significantly improved.(4)Electron microscopy results showed that sciatic nerve myelin of the saline treatment group appeared honeycombing,myelin and axonal separation layer stripping,with axonal thinning phenomenon.Compared with saline treatment group,miRNA-338 treatment group showed that the honeycomb and stripping were repaired by the tissue structure.The results showed that miRNA-338 had a significant effect on the repair of myelin sheath.(5)Immunohistochemistry showed that the expression of S100 and NF200 were higher in the miRNA-338 group than in the saline treated group,but the increase of S100 was more obvious.Coclusions: The results of this experiment showed that: 1.miRNA-338 could significantly improve the behavior score,nerve conduction function,histomorphology and other indexes of EAN rat model.2.provide new ideas for clinical treatment of autoimmune inflammatory diseases.