| Objective:The study investigated the effects of balance fat and its mechanism of lipid metabolism in mice and provided the experimental support and basis for the people balanced, moderate intake of fat.Methods:Eight-week old male SPF Kunming mice were randomly divided into nine groups with eight of each group. Rod-shaped compound feed was prepared for basal diet excepting oil by consulting nutritional requirements of GB 14924.3-2010,according to the normal fat intake and the actual intake of people convert into two doses in mice, and 3.8% and 6.5% blend oil of 1:1 of lard and vegetable fat were added in the diet respectively, namely Group A:Arowana oil group, group B:corn oil and lard group, group C:rapeseed oil and lard group, D group:soybean oil and lard group, I groups:control group (without added fat). Every Monday morning 9:00 weighing, after 8W, blood was collected for liver function examination, pathological autopsy was done and liver tissues were taken complete for calculating liver index and HE staining. Liver Junction, blood lipid and serum protein level were detected.The PPARa, PPARyand RXRaexpression were measured by Real-time quantitative RT-PCR in liver.Results:(1) Results of weight showed that compared with fat group,body weight of no fat group mice had a trend of rising. Compared with high dose group, bodyweight of recommended dose groups had a downward trend. Body weight of each group was no significant difference (P> 0.05). (2) Results of liver index showed that compared with no fat group,liver index of fat group mice had a trend of rising and blend oil of 1:1 of lard and corn oil increased significantly (P<0.05), and the the recommended dose of blend oil of lard and rapeseed oil or blend oil of lard and soybean oil significantly increased (P<0.01).Compared with high dose groups, the recommended dose group had a upward trend and blend oil of lard and soybean oil increased significantly (P<0.01). (3) Results of liver index showed that liver of groups were regular. (4) Results of liver function showed that compared with fat group,the no fat group mice had a rising trend,especially the level of ALP increased significantly (P<0.05).The level of ALT in blank group increased, relative to blend oil of cornoil and lard group increased significantly (P<0.05).The level of AST was up-regulated with blank group,relatively high doses of Arowana oil, blend oil of corn oil and lard group, the recommended dose of blend oil of Rapeseed Oil and lard groups increased significantly (P<0.05). The level of AST of the recommended dose of Arowana oil group relative to blend oil of corn oil and lard group, Rapeseed Oil and lard group were significantly increased (P<0.01). (5) Blood lip id results showed that the level of HDL in blank group relative to the recommended dose of Arowana oil, the recommended dose of blend oil of Rapeseed Oil and lard group, blend oil of soybean oil and lard group significantly decreased (P<0.01),and the recommended dose of blend oil of cornoil and lard were the highest. The level of TC and LDL with fat group, was increased relative to the blank group,and the high dose of blend oil of corn oil and lard group LDL level increased significantly (P<0.01). Relative to the control group, the level of TG in fat groups was increased,and high dose of blend oil of corn oil and lard group, the recommended dose blend oil of Rapeseed Oil and lard group increased significantly (P<0.01). The recommended dose of blend oil of Rapeseed Oil and lard compared high does of Rapeseed Oil and lard group, recommended dose of blend oil of soybean oil and lard group, corn oil and lard group, Arowana oil group increased significantly (P<0.01). (6) The level of serum protein results showed that there were no differences of ALP and TP levels in groups. The level of G in recommended dose of Arowana oil group relative to the blend oil of cornoil and lard group decreased significantly(P<0.05). The level of G in high dose of Arowana oil group is relatively the blend oil of Rapeseed Oil and lard group, soybean oil and lard group significantly decreased (P< 0.01),and a relatively high dose corn oil and lard group decreased significantly(P<0.05). (7) The results showed that the expression of PPARain blank group relative the recommended dose of groups significant increased (P<0.01). The expression of PPARain recommended dose of Arowana oil group relative to the blend oil of corn oil and lard group, Rapeseed Oil and lard group, soybean oil and lard group were significantly up-regulated (P<0.01).The recommended dose of Rapeseed Oil and lard group relative to corn oil and lard, soybean oil and lard group was significantly down regulated the expression of PPARa (P<0.01). In the blend oil of Rapeseed Oil and lard groups,soybean oil and lard groups, high dose significantly up-regulated the expression ofPPARa(P<0.01).Compared with recommended dose of fat groups (excepting blend oil of soybean oil and lard groups),the blank group PPARy level significant increased (P<0.01). In Arowana oil groups, blend oil of corn oil and lard groups, high dose groups significantly decreased PPARy levels (P<0.01).. At the recommended dose groups, soybean oil and lard group PPARy levels were significantly lowered (P<0.01).In high dose groups, blend oil of soybean oil and lard group PPARy levels significantly increased (P<0.01). The levels of RXRain blank control group relative to the recommended dose group (excepting corn oil and lard group) increased significantly (P<0.05). In the recommended dose groups, the blend oil of corn oil and lard significantly up-regulated RXRalevels (P<0.01). The blend oil of soybean oil and lard group significantly increased levels of RXRain high dose groups (P<0 .01).Conclusion:(1) Dietary no fat can lead to body weight, liver index increased, liver function decreased, blood lip id levels below normal, increase expression of PPARa and RXRa mRNA in liver, reduce expression of PPARy mRNA in liver, do damage to human health(2) The recommended dose of dietary fat will moderate weight, liver index increased, liver function be normal (excepting Arowana oil and blend oil of Rapeseed Oil groups), blood lipid levels (excepting blend oil of Rapeseed Oil group), down regulate the expression of PPARa and RXRa mRNA in liver, increase liver PPARy mRNA in liver, benefit to human health.(3) Excessive dietary fat can lead to weight loss, liver index, liver function decline, blood lipid level increase, increase expression of PPARa and RXRa mRNA in liver, reduce expression of PPARy mRNA in liver, do damage to human health.(4) The recommended dose of blend oil of corn oil and lard, soybean oil and lard combination weight, liver index,liver function were normal, blood lipid level were moderate, the liver PPARa were low expression, PPARy was higher expression, were the more optimal combination of the four balance fat combination of Arowana oil, blend oil of corn oil and lard,rapeseed oil and lard, soybean oil and lard group. |
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