Study On The Preparation Technology And Quality Standard Of Scutellaria Radix Total Flavone Aglycone Extract And Androgphis Herba Extract

This paper can be divided into three parts:The first part describes the study on preparation technology and quality standard of Scutellaria Radix total flavone aglycone extract, the second part describes the study on preparation technology and quality standard of total lactones in Androgphis Herba, the third part is the summarize about progress in the research of Scutellaria Radix, Androgphis Herba and their extracts.The first part describes the study on preparation technology of Scutellaria Radix total flavone aglycone extract. By pH, Scutellaria Radix enzyme liquid were extracted particle size,decocting conditions (water content, time, number), enzymatic time, enzymatic pH value, to determine the best preparation technology:Scutellaria Radix meal crushed 10 mesh, added 10 times amount water,the solution was djusted pH3.0 by HCl,stired 0.5h, and filtered,dregs were put into 12 times the amount of boiling water, adjusted pH6, decocted twice and each time 0.5 h, and filtered,added Scutellaria Radix enzyme liquid, mixed and adjusted pH6,enzymed for 6 h at 50 "C.Refering to the Chinese Pharmacopoeia 2015 Edition (a) of tanshinones and salvia total phenolic acids quality standards to Scutellaria Radix extract of character, differential diagnosis, examination, determination for a more comprehensive study. Water, residue of ignition, heavy metals, macroporous resin residue were examined; The HP1C method was established by determination of Scutellaria Radix total flavone aglycone extract. According to the determination results, the product was calculated on dry goods, the contents of sumation of baicalein, wogonin,oroxylin A were not less than 65.0%,total flavonoids were not less than 73.0%.The secend part adopted the methods of diacolation extraction and macroporous resin purification prepared by Androgphis Herba. Confirmed the optimum diacolation extraction process by the orthogonal experiment. Types of macroporous resin, volume of eluent and ethanol concentration were observed by using the single factor investigation method. The results were as follows:Androgphis Herba crushed 10 mesh, percolated at 4 mL/minkg with 70% ethanol, collected 10 times amount of percolating liquid, recovered ethanol to tasteless.filtrated and got tanshinoneolution, volume of eluent and ethanol concentration were observed by using the single factor investigation method. The sample solution was purified with HPD100 macroporous adsorption resin, washed with 2 BV water, and 3 BV 25% ethanol was used eluting impurity, eluted with 3 BV 70% ethanol. The eluent was collected and recyced to dense paste,tanshinone precipitation was added, Dissolved in 95% ethanol and refluxed 20 min, filtrated, added 2% activated carbon in the filtrate, reflowed 20 min, filtrated, the recovery of ethanol, concentrated, decompression drying and got it.Refering to the Chinese Pharmacopoeia 2015 Edition (a) of quality standards to extract of character, differential diagnosis, examination, fingerprints, determination for a more comprehensive study. Water, residue of ignition, heavy metals were examined; Androgphis Herba fingerprints was established by studying of the mobile phase, wavelength, column temperature, flow rate. Evaluated by similarity of chromatographic fingerprint evaluation system (2004A), the fingerprint similarity was greater than 0.90; According to the determination results, the product was calculated on dry goods, the contents of sumation of androgapholide, neoandrogapholide, deoxyandrogapholide, dehydandrogapholide was not less than 35.0%.To sum up, this paper studies preparation technology and quality standard of Scutellaria Radix total flavone aglycone extract and Androgphis Herba.The method is simple feasible, quality control, and quality control to provide the reference for the proprietary Chinese medicine application.