Study On Distribution And Pharmacokinetics Of Fe₃O₄ Nanoparticles In Mice

Fe₃O₄ nanoparticles as excellent functional materials, which have been widely applied in the field of biomedical research, especially the advantages of diseases diagnosis and treatment have drawn researchers attention. This topic mainly probed into Fe₃O₄ nanoparticles with different size and surface modification about absorption, distribution and metabolism in the mice. The study on Fe₃O₄ nanoparticles provides safety evaluation of experimental basis in biomedical field.1. Study on the kinetics of Fe₃O₄ nanoparticles24 kunming male mice were randomly divided into four groups, each group of 6, respectively,normal saline control group,26 nm experimental group,34 nm experimental group and 81 nm experimental group. According to the 5 mg/kg mice weight,0.2 ml of Fe₃O₄ nanoparticles aqueous solution ?concentration of 500 ug/ml? were injected by mice tail vein, taking the tail blood in accordance with each time point, The content of iron in mice's blood was detected by coupled plasma emission spectrometry ?ICP-OES? when before injection, after injection 5 min,10 min,15min,30 min,45 min, 1h,6 h, 1 d,3 d,5 d. Results showed that the content of iron in mice's blood was up to maximum after tail vein injection 5 min. The results showed that the concentration-time curves conformed to two-compartment model.2. The biochemical indexes changes in the mice serumAfter injection of 1 h,5 d,28 d,50 d,Taking blood sampling used to centrifugal separation, The mainly serum biochemical indexes in mice blood sampling were tested by automatic biochemical analyzer test. The blood biochemical indexes such as glutamic oxalacetic transaminase ?ALT?, lutamic-pyruvic transaminase ?AST? levels rise, urea nitrogen ?BUN?, creatinine ?CREA?and total cholesterol ?CHOL? content have no obvious changes, the change said Fe₃O₄ nanoparticles has slightly on the liver and kidney damage to mice.3. The distribution of Fe₃O₄ nanoparticles in miceFe₃O₄ nanoparticles solutions was injected by mice tail vein, the mice were put to death by cervical vertebra when before injection, after injection 5 min,10 min,15min,30 min,45 min,1h,6 h,1 d,3 d,5 d. The content of iron in mice's tissues such as liver, spleen, heart, lungs and kidneys, were detected by coupled plasma emission spectrometry ?ICP-OES? according to all the time points. Results show that the distribution of Fe₃O₄ nanoparticles in mice, in turn, for liver, spleen, heart, lungs and kidneys, and the molecular weight distribution quantity varies with the size and surface modification of Fe₃O₄ nanoparticles.4. Histopathologic observation of tissues in miceThe sections of tissue in heart, liver, spleen, lungs and kidneys were observed by histopathology HE staining after injection 1 h and 5d. The results shows that Fe₃O₄ nanoparticles has no evident pathological damage to various organs in mice under this experiment condition. The histopathological changes may be related to experimental animals, the size, the dose and action time of Fe₃O₄ nanoparticles.Anyhow, the distribution and metabolism process of Fe₃O₄ nanoparticles within the body were rather complex, which was affected from the body's circulatory system, immune system and a series of metabolic mechanism. The concentration-time curves of Fe₃O₄ nanoparticles in the mice conformed to the pharmacokinetic double chamber model, mainly distribution in the spleen and liver.From the mice serum biochemical indicators were tested by automatic biochemical analyzer and histopathological examination,we can determine the toxic effect of target organ was the liver and kidney, metabolic pathway was mainly the liver and kidneys.