| Colorectal cancer is the top five incidence and mortality of malignant tumor in China, which does great harm to the health of citizens. To study the molecular pathogenesis of colorectal cancer and searching genetic marker locis for early warning is imminent.Our team found DDI2 carried a LOF site in a sCRC case, and finally identified it as an important candidate tumor suppressor gene through literature review and analysis of the biological functions, and already affirmed DDI2 may reduce proliferation and migration of colorectal cancer cells in vitro. This study have done a further research minly in vivo on the original basis. Western Blot showed DDI2 expressed in all of the colorectal cancer cell lines, which supprted the veracity of experiment in vitro. Immunohistochemical method found there was a lower expression of protein in colorectal cancer tissues, prompting that DDI2 might have the function of antitumor. Tumor formation in nude mice found tumor of experimental group growed significantly slower than the control group, indicating that DDI2 could significantly inhibit the growth of colorectal cancer cells in vivo. Combined all of our results, we confirm that DDI2 can inhibit cancer cell growth in colorectal cancer genesis and development significantly.Subsequently, we found STX1A, which may can conbine with DDI2, from the reference that VSM1 can combine with SSO1 in yeast. We constructed the expression vector of its wide type and truncature type, made a desk study of the combination of DDI2 and STX1A. Real-time fluorescent quantitative PCR showed that both DDI2 and STX1A had mRNA expression in colorectal cancer cell lines, which can provide the premise of the combination of the two genes. Western Blot also showed both DDI2 and STX1A had expressed in experimental cells used in co-immunoprecipitation. But we didn't get specific result that DDI2 can conbine with STX1A in co-immunoprecipitation, more sensitive testing also need to notarize the combination of DDI2 and STX1A. |
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