Imaging Human Gastrointestinal Muscular Layer In Transverse And Longitudinal Sections Based On Multiphoton Microscopy

The variation on the morphology and distribution of the main components in muscular layer is very relevant to the pathology in gastrointestinal tissue. The muscular layer is a boundary between T1 and T2 cancer staging, it have important significance for cancer detection and diagnosis. In recent years, multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) has already been widely used in biological tissue without the need of additional fluorescent dyes. In this paper, firstly, we used MPM to investigate the microstructure changes of normal and T2 cancer staging human gastric muscular layer in transverse and longitudinal sections. Our results demonstrated that MPM can clearly reveal the high-resolution images of human normal and T2 cancer staging gastric muscular tissue at the molecular level. The morphological characteristic parameters of cancerous human gastrointestinal tissue changed significantly compared with that of healthy ones; the morphological characteristic also varied when compared with muscular layer tissues of transverse and longitudinal. On this basis, we used MPM to obtain the high-resolution MPM images of human normal and cancerous human colorectal muscular tissue in different sections. We qualitative analysis the morphology microstructure characteristics of muscular tissue, and measure the collagen content to further quantitative analysis experiment results. These features are well consistent with hematoxylin and eosin (H&E) stained images. Our results demonstrated that MPM can bring more detailed complementary information on tissue architecture through observing transverse and longitudinal sections of human normal and T2 cancer staging gastrointestinal muscular tissues. More importantly, MPM imaging technology has great potential for providing more accurate and comprehensive guidance on clinical treatment and management of gastrointestinal tumor. With the development of miniaturization of multiphoton endoscopy, MPM technology has the potential to provide real-time nondestructive histopathological diagnosis for gastrointestinal tumor.