Effects Of Exogenous Catalase On Tissue Antioxidant Capacity And Intestinal Microflora

Dietary nutritional imbanlance will cause the body's REDOX state imbalances, produce excessive reactive oxygen free radicals(reactive oxygen species, ROS), change the intestional flora structure and induce a variety of chronic diseases. So how to alleviate the oxidative stress induced by dietary is an urgent problem to solve. This research studies on the abiltity of catalase to alleviate intestinal oxidative stress and improve the intestinal flora structure. There are two parts in this research.(1) The influence of catalase on the intestinal redox state and the flora. This experiment added different levels of catalase in high-fat diet mice, by detecting T- in the ileum AOC, T-SOD and CAT REDOX level to evaluate the experimental animal intestinal REDOX state. The intestinalflorawas analysised by T- RFLP method. LPS, IL- 6, IFN- gamma and TNF alpha are determined to examine the effects of catalase on inflammatory cytokine levels in serum and tissue of grout. Analyzing the above contents comprehensively, we explored whether adding hydrogen peroxide enzyme in food can realize the antioxidant effect.The results show that after different intake of fat, mice's weight are different. Body weight in high-fat group isobviouslyhigher than that of normal group. After the hydrogen peroxide enzyme intervention, the effects of high-fat diet on body weight reduced.But the body weight is still higher than the normal group. Body fat rate in high fat group is obviously higher than that of normal group.Body fat ratein catalaseintervention group is between normal group and high fat group, but no significant difference between different doses of intervention group.Compared with the normal group, high-fat group's T AOC, T- SOD, CAT, GSH and gsh-px levels are significantly reduced. Compared with high-fat group, intervention group's T AOC, catalase T-SOD, CAT, GSH and gsh-px levels are higher than high-fat group. Compared with the three intervention groups, we find that the highest increase of intervention group is NO.2, followed by NO.1. The intestinal flora results of five experimental group are different, where T-RF60 is appeared in high fat diet group and intervention 1, 2, 3 groups, not in the normal group; T-RF93 is appeared in the intervention group; T-RF199 is onlyappeared in the normal group and the high fat group; T-RF587 and T-RF425 are not appearin the high fat group. Among these floras, T-RF425 and T-RF587 are beneficial. T-RF220 is disadvantageous to the human body. So catalase can improve the adverse effects of high fat dietto a certain extent.(2) The influence of catalase on vitro intestinal flora. Stool samples collected from 3 volunteers were inoculated into GAM broth with different contents of catalase.Then structural survey of the microbiota of these samples wasdetermined by high throughput sequencing of the V3 region of the 16 S ribosomal RNA gene.The result show that addition of catalase has significant effect on structural of gut microbiota of samples. The variation is caused by Clostridium, Prevotella, Allisonella, Megamonas, Escherichia/Shigella, Collinsella, and Bifidobacterium. Meanwhile, the addition of catalse alsopromotesthe growth of Escherichia/Shigella, Lactobacillus, Lactococcus, Allisonella, and Megasphaera, and inhibit the growth of Enterococcus and Bifidobacterium.The addition of exogenous catalase can improve the intestinal antioxidant capacity, reduce the inflammatory response and promote the structure of intestinal flora in high fat diet mice.