Study Of ABCA1 Expression In The Formation Of Atherosclerosis In Patients With SLE

SLE(Systemic lupus erythematosus, SLE) is a multisystem damage of chronic Systemic autoimmune inflammatory connective tissue disease, with a variety of anti-bodies inthe patients' serum, including anti ABCA1 autoantibodies s.ABCA1 is a membrane proteinthat promoted the transfer of the intracellular cholesterol outflow, phospholipids and inhibited inflammation of blood vessel walls and promoted the integration of high density lipoprotein cholesterol(hdl-c) generated by combined with Apo A 1.The expression of ABCA1 plays an important role in the transport of cholesterol. In this study, a western blot detection method of ABCA1 autoantibody was established. In the base of existing ABCA1 expression adjust filter model, rutaecarpine was chosen to research ABCA1 autoantibodies in vitro of intracellular cholesterol outflow, and the influence of ABCA1 expression level in patients with SLE and the correlation of formation of atherosclerosis, which discussed the ABCA1 protein expression in lupus effects of early atherosclerosis, to guide clinical early prevention of atherosclerosis in patients with SLE. Objective:Analyse the role of anti ABCA1 autoantibodies in the formation of atherosclerosis in patients with SLE and examine the effect of rutaecarpine in improving autoimmune antibody ABCA1 antibody with ABCA1 protein expression level in SLE. Expound the mechanism of atherosclerosis induced by anti ABCA1 antibody to provide theoretical basis for the prevention and treatment of early atherosclerosis in patients with SLE.Method: Comparison of serum lipid levels between 80 SLE patients and healthy subjects from January 2014 to June 2015 in Rheumatic immunology of the First Affiliated Hospital of Zhengzhou University.Human monocyte cell line THP-1 cells were cultured in vitro and stimulated by PMA to express the high expression of ABCA1 protein. The ABCA1 protein was extracted and identified.The human Ig G antibody was purified by protein A gel chromatography column, and the effect of Ig G on cholesterol efflux in ABCA1 cells was detected by Ig G antibody in vitro.Real-time quantitative PCR and Weseren blotting were used to detect the transcription level of ABCA1 m RNA and protein expression of ABCA1. Rutaecarpine selected and used to study the SLE patients with ABCA1 antibody positive group ABCA1 antibodies in vitro on intracellular cholesterol efflux in SLE patients and the correlation between ABCA1 expression level and atherosclerosis formation.Result: The positive rate of ABCA1 antibody in SLE patients was significantly higher than that in healthy subjects, and the difference was statistically significant(P<0.05). The positive rate of ABCA1 antibody in SLE with atherosclerosis group was higher than that of SLE without atherosclerosis group, and the difference was statistically significant(P < 0.05). The cholesterol efflux rate of anti ABCA1 antibody positive IgG intervention group was lower than that of the Ig G intervention group, and the difference was statistically significant(P < 0.05). MRNA in the treatment group was up to 0.49 ± 0. 05( P<0. 05) in the treatment group ABCA1( 5.0?g·m L-1). The treatment group of Rutaecarpine and anti ABCA1 antibody was raised 0.52 ± 0.01(P<0. 05) compared with the control group of anti ABCA1 antibody. There was a significant correlation between the expression level of ABCA1 protein and ABCA1 antibody in patients with SLE, and Rutaecarpine can significantly increase the expression of ACBA1 protein in SLE patients, which can promote the cell cholesterol efflux.Conclusion: Compared with the normal people, the positive rate of anti ABCA1 antibody in the serum of SLE patients was significantly higher. The cholesterol efflux rate of THP-1 cells in vitro was significantly higher than that of normal people after purified anti ABCA1 antibody positive SLE patients. That means anti ABCA1 antibody has inhibitory effect on the efflux of intracellular cholesterol in patients with SLE and may promote the formation of atherosclerosis. ABCA1 expression plays a key role in the occurrence of atherosclerosis in patients with systemic lupus erythematosus. The improving of ABCA1 expression level in SLE patients can effectively increase the cell cholesterol efflux.