Complete Genome Sequence Of Gordonia Bronchialis&Structural And Functional Analyses Of Its Lipoglycan

Gordonia generally exists in the environment.Some species with the powerful creature of the repair ability can be used in the management of soil and water pollution;other species are reported to infect immune deficiency disorder and patients with normal immune system.But there were few reports about the research related on its pathogenic mechanism,respiratory diseases it cause are easy to be misdiagnosed as tuberculosis.So it is significance to research this kind of pathogens for clinical diagnosis and treatment.This research focus on Gordonia bronchialis clinical strains,in which the genome structure and function of the specific antigen lipoarabinomannan were determined.The results of the study are as follows:(1)Gordonia bronchialis clinical strains(named: WH31)was isolated from the sputum specimens of patients clinically diagnosed as tuberculosis with diabetes at Wuhan Medical Treatment Center in October 2009.Isolate WH31 genome was sequenced with Gordonia bronchialis 3410 T as the reference genome.This study analyzed the physicochemical characteristics and pedicted the functions of Grodonia bronchialis mmpL3.The results show: Many variations were found in the comparative genome analysis.Although gene deletion was found in WH31,the isolate was still ability to survive in the host.The gene(Gbro4481)which has two MMPL domains and a Sterol-sensing domain is found homologous to Mycobacterium tuberculosis mmpL3,and is predicted to involve in the lipid metabolism,signal conduction and material transportation.The results provide the foundations for pathogenic research of the acid-fast strain and looking for durg target.(2)The lipoglycans of clinical isolates WH31 and Mycobacterium tuberculosis(MTB),No-tuberculosis Mycobacterium(NTM)were ectracted.Then crude extractions were purified with the AKTA purification system(Sephacryl S-100 HR).After PMP derivatization,high performance liquid chromatography(HPLC)analysis was determined for the molecular mass of the purified LAM.The strains and purified LAM were used to induce DC,then secretion of cytokines IL-6 were detected by ELISA.The results show: Gbr LAM was purified from WH31 cell wall by AKTA system.And GbrLAM is composed of arabinose and mannose of which ratio is 3:1.The molecular mass of WH31 LAM is ~8 kDa.The cytokine secretion analysis found that WH31 can not significantly induce the dendritic cells to secret IL-6 while MTB and NTM can do it well.The comparative analysis of MTB,NTM and Gordonia bronchialis clinical isolates LAM could provide the foundation to reveal the relationship between different pathogens LAM,pathogenicity and virulence.