Effective Of The Different Intensity Forcible Thrusting Of The Needle Exerted On Acute Closed Soft Tissue Injury Of Rabbit

Objective To observe the different intensity forcible thrusting of the needle exerted on acute closed soft tissue injury of rabbit the effect of 5-hydroxytryptamine (5-HT)?prostaglandin E2 (PGE2) and pathology.Method Using Chinese long-eared rabbits, weight 2.5-3 kg from animal experiment center of zhejiang Chinese medicine university, before the Chinese long eared rabbit of model adaptive feeding for one week,100 successful China long ear rabbits model randomly divided into control group and experimental group A (250 g), experimental group B (500 g), experimental group C (1000 g) and experimental group D (2000 g), each group of 20. According to the time of when it's killed (pressure after 10 min, the second day, the fourth day and the eighth day) is divided into 1,2,3,4 four subgroups; Namely the control group 1, the experimental group A1, B1, C1, D1, each group of five. All the successful model animals before the intervention and after to be put to death get the ear edge venous blood around 4 ml used in the detection of 5-hydroxytryptamine. Clipping executed rabbit hit the center of the area and cyanosed muscle tissue, was doing hematoxylin-eosin staining for pathological observation and prostaglandin E2.Results All of experimental group can reduce the content of 5-hydroxytryptamine in peripheral blood, and no statistical difference between each groups. The whole experimental group were decreased the content of prostaglandin E2, but in group A and group B is better than that of group C and group D. There is no statistical difference between A and B, also no statistical difference between C and D. Each experimental group can't improve pathological indexes of acute closed soft tissue injury of rabbit, and with no significant difference between control group and each group.Conclusion Different intensity was exerted on the model of acute closed soft tissue injury of rabbit has adjusting effects of 5-hydroxytryptamine and prostaglandin E2,250 g and 500 g pressure group curative effect is more significant. Several different intensity forcible thrusting of the needle does not improve acute closed soft tissue injury of rabbit pathological indicators.