The Interaction Between MMS2 And P53 On Proliferation And Apoptosis Of Colon Cancer Cells

ObjectiveTo explore the relationship between MMS2 and P53 in colon cancer cell(THC-8307) and the regulatory effects of proliferation and apoptosis in THC-8307 by MMS2 and P53.MethodsWe experimentally suppressed the MMS2 and P53 expression in human colon cancer cells by the interference RNA technology(RNAi) as monitored by RT-qPCR and Western blot.THC-8307 cells that express rate significantly reduced were elected as case group,while using untreated cells as the blank control group, and mock-treated cells as the negative control group. After separately interfering the target genes in each group, test the relationship and expression level of the two genes. Utilizing flow cytometry techniques to test cells proliferation and apoptosis rate of each group.ResultsCompared with the two control groups, colon cancer cells in which MMS2 were silenced displayed significant increase of P53 mRNA and protein levels(P<0.05). Meanwhile, Compared with the two control groups, colon cancer cells in which P53 were silenced displayed significant increase of MMS2 mRNA and protein levels(P<0.05).No significant differences between the two control groups(P> 0.05).MMS2-depleted cells displayed increase in apoptosis rates, for both early and later stages(P<0.05),While P53-depleted cells and control groups had no statistic difference( P> 0.05).ConclusionsMMS2 and P53 negatively regulate each other to some extent in colon cancer cells and both of the two genes co-regulate proliferation and apoptosis to a certain degree in colon cancer cells,MMS2 gene induced colon cancer cell(THC-8307) apoptosis may be through the activation of P53.