Preliminary Study Of The Application Of Targeted Gold Nanopartilces In Imaging And Radiotherapy

Purpose:To explore the synthesis method of the multifunctional molecular probe AuNPs-Tf-AuNCs. And to discuss the application of the probe in the CT targeted imaging and radiation enhancement.Methods:Synthesize the multifunctional molecular probe AuNPs-Tf-AuNCs through oxidation reduction method, test the bio-safety and cell targeted CT imaging detection based on the morphological and functional characterization. At the same time, explore the probe in increasing radiotherapy sensitivity and the applications in molecular imaging.Contents:(1) Using the classical oxidation reduction method to synthetic AuNPs whose grain size is about 13 nm, and doing the hydrophilic modification. In the assurance of AuNPs physical and chemical properties of stable under the premise, to investigate the biological safety and whether it can connect target material and the possibility.(2) With Tf as a stabilizer and reductant, synthesis the gold nanoclusters inside the Tf---Tf-AuNCs, and explore the target, biological safety and fluorescence imaging function,and to verify Tf-AuNCs the ability to enhance the radiation sensitization.(3) Link the synthesized and modified AuNPs and Tf-AuNCs probes by polyethylene glycol(PEG) and here comes the AuNPs-Tf-AuNCs,which process targeting, CT imaging, radiotherapy sensitization of multifunctional molecular probes.Characterize the probe in morphological and function.(4) Explore the multifunctional molecular probes AuNPs-Tf-AuNCs on cell survival rate and cell radiation therapy effect, and calculated the cell survival rate,comprehensive evaluation multifunctional molecular probes AuNPs-Tf-AuNCs in CT imaging and radiotherapy enhanced sensitivity effect.Results:(1) 13 nm and particle size of AuNPs Tf-AuNCs were successfully prepared by a simple safety and green method. The results showed that 3nm with good dispersion,simple synthesis method, good repeatability and there is no obvious agglomeration.(2) AuNPs molecular probes were successfully prepared by using PEG andTf-AuNCs as coupling agents and AuNPs-Tf-AuNCs molecular probes were successfully prepared by the amide linkage. The results showed that the AuNPs-Tf-AuNCs molecular probe has uniform particle size, good dispersion, and the size is about, 60 nm which has the condition of molecular imaging cell and in vivo experiment.(3) In this study, the MTT method was used to detect the toxicity of AuNPs-Tf-AuNCs molecular probe. The results showed that the synthesized probe AuNPs-Tf-AuNCs and its components have good biological safety.(4) AuNPs-Tf-AuNCs molecular probe in the original AuNPs based on the increase of Tf-AuNCs, not only to enhance the ability of the probe as a CT contrast agent, but also with a good target.(5) AuNPs-Tf-AuNCs can be used as a fluorencent probe agent on the PC3 which possess more TfR of their cell surface.(6) Because of the Tf-AuNCs,the ability of the whole probe AuNPs-Tf-AuNCs in radiotherapy was enhanced, AuNPs-Tf-AuNCs is a multifunctional probe which combine the CT imaging and radiotherapy.Conclusion:AuNPs-Tf-AuNCs molecular probe was prepared in this study has simple synthetic method, CT imaging capability is excellent, excellent safety, also can enhance cell radiotherapy sensitization, in the study of molecular imaging in vivo imaging has a good application prospect.