The Role Of PKM2 In The Pathogenesis Of Endometrial Carcinoma And Its Mechanism

Objective Explore PKM2 role in the occurrence and progress of endometrial carcinoma and its possible mechanism of action, to illustrate mechanism of endometrial carcinoma experimental basis, at the same time provide new targets for the treatment of endometrial carcinoma.Methods 1. Collect 60 cases of endometrial cancer patients(group A) and 60 cases of endometrial atypical hyperplasia patients(group B) which from the first Central Hospital of Tianjin city and Tianjin Jixian County People's Hospital from 2010 January to 2014 October as the research object,64 patients with normal proliferative endometrium(Group C) were used as the control.Improve the clinical data, including patient age, maternal inferior), pathological data(tissue types, grading, staging, etc.), clinical treatment and prognosis, treatment, metastasis and recurrence, etc.). 2. The expression of PKM2, p-PKM2 and HIF1? in the tissues of each group were detected by immunohistochemical method. 3. The statistical analysis to make sure the sugar metabolic abnormalities in endometrial cancer progress plays an important role, its action can be expressed through raising HIF1? and promote cell proliferation and tumor progression.Results 1. Comparison of three groups of patients: There was no significant difference between the three groups in the age of the patients(F=1.498, P=0.227). 2. PKM2 expression: In group A, group B and group C, The positive expression rate of PKM2 in the normal group(group C) was the lowest, while the expression rate of PKM2 in endometrial carcinoma group(group A) was the highest. Three groups of endometrial tissue cells PKM2 expression positive rate of 86.7%,63.3% and 40.6%,the difference was statistically significant(chi-square=28.179,P<0.001). The positive rate of PKM2 expression in endometrial carcinoma group was significantly higher than that in the normal proliferative phase(chi-square=28.132,P<0.001) and the endometrial atypical hyperplasia group(chi-square=8.711,P=0.003). The positive expression rate of PKM2 in the endometrial atypical hyperplasia group was significantly higher than that in the normal proliferative phase group(chi-square=6.394,P=0.011). 3. p-PKM2 expression:In group A,group B and group C, The positive expression rate of p-PKM2 in endometrial cancer group was the highest, while the p-PKM2 positive expression rate was the lowest in the normal proliferative phase endometrium group. The positive expression rates of p-PKM2 were 73.3%, 53.3% and 34.3%,the difference was statistically significant(chi-square =18.881,P<0.001). The positive expression rate of p-PKM2 in endometrial carcinoma group was significantly higher than that in the patients with endometrial atypical hyperplasia group(chi-square =5.167,P=0.023) and normal group(chi-square=18.879,P<0.001). The positive expression rate of p-PKM2 in the endometrial atypical hyperplasia group was significantly higher than that in the normal proliferative phase group(chi-square=4.528,P=0.033). 4 HIF1? expression: In group A,group B and group C,The positive rates of HIF1? expression were 83.3%, 63.3%, 43.8%, respectively, and the difference was statistically significant(chi-square=20.829,P<0.001). The positive expression rate of HIF1? in endometrial cancer group was significantly higher than that in the patients with atypical hyperplasia(chi-square=6.136, P=0.013) and normal group(chi-square=20.793, P<0.001). The positive expression rate of HIF1? in endometrial atypical hyperplasia group was significantly higher than that in the normal endometrium group(chi-square=4.770,P=0.029). 5. Correlation analysis of expression level of PKM2,p-PKM2 and HIF1? in three groups of patients: In the endometrial tissue of the endometrial carcinoma, endometrial atypical hyperplasia and normal proliferative phase,the expression level of PKM2 was positively correlated with the expression level of HIF1?, the correlation coefficient was 0.463, P=0.000, the expression level of PKM2 was positively correlated with the expression level of p-PKM2, the correlation coefficient was 0.466,P=0.000,the expression level of HIF1? was positively correlated with the expression level of p-PKM2, the correlation coefficient was 0.313,P=0.002. 6. Relationship between expression of PKM2, p-PKM2 and HIF1? in patients with clinical pathological characteristics in endometrial cancer:There were no correlation between PKM2, p-PKM2 and HIF1? expression with age(P = 0.601, P = 0.119, P = 0.296); tumor tissue study(P = 0.497, received operations, P = 0.215) type; the degree of tumor differentiation(P=0.788, P=0.680, P = 0.221); primary tumor size(P = 0.591, P = 0.099, P = 0.224) and tumor clinical staging(P=0.874, P = 0.691, P=0.946).Conclusions The sugar metabolic abnormalities in endometrial cancer progress plays an important role, its action can be expressed through raising HIF1 a and promote cell proliferation and tumor progression.