| Exposure or excessive absorption of arsenic can cause a variety of diseases and pathological damage. Experiments were carried out to investigate the effect of arsenic trioxide treatment on mice kidney;the direct efffct on human endometrium stromal cells. [Methods]: Six-week-old male mice were randomly divided into six groups with different doses of ATO(0, 0.3, 1.0, 3.0, 6.0, 9.0 mg/kg B.W), respectively. A continuous infusion of 5 days, 2 days off,emplement for 3 weeks. The experiment was repeated three times. Part one: To study the influence of different concentrations of ATO on kidneysof male mice – include the contents of serum creatinine and urea nitrogen,the kidney coefficient, kidney pathological changes. Part two: The Malondialdehyde MDA contents and superoxide dismutase SOD activity in the kidney of male mice were measured by test kits.In order to exposure of mice to explore the renal oxidative arsenic trioxide stress. Part three: To get the reslts of methionine sulfoxide reductase as an important antioxidant enzymes changes in the male mice kidney exposed to arsenic trioxide. The experimental design four methionine sulfoxide reductase(Methionine Sulfoxide Reductase, Msr) gene primers.The expression of Msr A、Msr B1、Msr B2 and Msr B3 genes were analyzed by quantitative real-time PCR.And the results were compared with the results of oxidative stress, explore the relationship between oxidative stress and the expression of methionine sulfoxide reductase.After the human endometrium stromal cells adherent growth, when the cell on exponential growth phase.add different doses of ATO(0, 1.0, 2.0, 3.0, 4.0 and 5.0 μmol / L). Part four: The direct impact of arsenic trioxide on human endometrial stromal cells were few researched,this study was designed to observe the Human endometrium stromal cells’ sensitivity of As2O3. Microscope to detect cell growth morphology,and cell viability curve after 48 hours of arsenic exposure. To make early exploration of arsenic trioxide’s estrogen-like effect. [Results]: The results showed that different doses of ATO has no significant effect on body weight and kidney coefficient.Serum creatinine and urea content has decreased; high dose group constitute damage to the kidney tissue.No significant difference in MDA content was observed among mice kidney treated with different doses of ATO. However, ATO treatment induced the increase of SOD activities in kidney, except 9.0 mg/kg ATO. Furthermore, different Msr genes showed differential responses to ATO stress. The expression of Msr A and Msr B3 genes were not affected by ATO treatment. ATO treatment resulted in the down-regulation of Msr B1 expression while the expression of Msr B2 gene was up-regulated.Effect of human endometrium stromal cells treated with different doses of the ATO is to promote the growth of low-dose and high-dose inhibition of growth, and cell damage. [Conclusions]: Exposure to arsenic in mice kidney tissue damage rendering dose dependent.It was suggested that ATO stress induced the increase of SOD activity and Msr B2 gene played an important role in ATO stress in mice kidney. Low concentrations of arsenic trioxide have hormone-like effects. |
