| Objective By using the technique of colloidin embedding prepared cerebral serial celloidin sections, the sectional anatomy and three-dimensional reconstruction of cerebral midline structures show the morphology of the cerebral midline structures and their adjacent relations. By using the Amira software can also choose a single structure or more structures to observe in multi-angle and multi-dimensional, and provide the morphological basis for surgical approach of resection of brain deep tumor. At the same time, the MRI cerebral images and Amria software were combined to measure the distance between the key points of the midline structure of the cerebral which can provide quantitative indicators for the resection of the third ventricle tumors.Methods 1. Selecting 14 cases of skull of adults which fixed by formalin and no obvious trauma and lesions(7 males, age range 52-82 years old, the average was 69.9 years. 7 cases were female, age range 53-88 years old, average for 72.4 years). Along the line which circled by the point of lateral canthus above 2.0 cm, external acoustic pore above 3.5 cm and occipital protuberance open the skull with chainsaw(KINZO25C708, Germany), then separate the dural,exposure cerebral sulcus, remove the nerves, blood vessels and pia mater layer by layer, and finally the cerebrums were removed. 2.After being flushed with 24 h, the brain samples were dehydrated by different concentration gradient(70%, 80%, 95%, 100%) of ethanol solution for step by step increasing, and then dipped by different concentrations of collodion solution(step by step from the low concentration of 5% increments up to 15%), finally with 15% of the collodion bag buried, volatile, curing, block. L-type Germany R.Jung(AG) was used to carry out serial sections and the thick is 500 um. 3.Using the OLYMPUS DIGITAL CAMERA SP-610 UZ to collect images, the images were saved as 4288 x 3216 specifications, JPG format. Using Amria software make image registration and segmentation, at the same time semi automatically outline the target structure for 3D reconstruction. Selecting 10 cases of coronal and horizontal specimens which used for the reconstruction and measurement of the third ventricle.4.MRI scan was performed in 25 male and female volunteers. The Dicom3.0 image was imported into fastview Syngo image processing system, and the images were stored in JPG format. The key points of the image are registered and reconstructed with Amria software, and the structure of the image is displayed in the Amria software, and then the Length 3D of the Measure module is used to measure the distance between the spatial structures. At this point, the distance measured is the pixel value, therefore, it must be converted to the value of the numerical value of MM.Results: 1. Observation and 3D reconstruction of the celloidin slices of cerebral. Coronal observation: From the frontal lope to the occipital lobe do continuous slices.The layer of the corpus callosum and the anterior horn of the lateral ventricle was defined as the first layer. 35 level appeared anterior commissure, the thalamus appeared in 54 levels, the medial wall of the thalamus constitute the lateral wall of the third ventricle, lentiform nucleus and thalamus separated by the posterior limb of the internal capsule. Between the column of fornix and anterior thalamic nuclei have foramen of Monro. The interthalamic adhesion connected both sides of the thalamus. Horizontal observation: From the frontal lope to the bottom of the cerebral do continuous slices. I The layer of appearing the corpus callosum was defined as the first layer. Continuing to observe, immediately the fornix appeared, the top of the third ventricle is positioned at the beneath of the fornix. Continue down observation, you can clearly see the Y shaped intramedullary plate perpendicular to the the dorsal thalamus.Between the column of fornix and anterior thalamic nuclei have foramen of Monro which connected the third ventricle and the lateral ventricle. The anterior commissure appear in the front of fornix column. Sagittal observation: From the temporal lobe to the cerebral longitudinal fissure do continuous slices. Apearing of the putamen level is defined as the layer 1 and continue to observed from the outside to the inside, in the 10 th level appeared the globus pallidus, and then we can see the corona radiata and pulvinar. In the 21 st level, the caudate nucleus appeared which located in the top of the putamen and the globus pallidus. The caudate nucleus located above the body of the lateral ventricle. Anterior limb of the internal capsule located between the caudate nucleus and lentiform nucleus and posterior limb of the internal capsule situated between the lentiform nucleus and dorsal thalamus. 2. 3D reconstruction and volume measurement. The results of the three- dimensional reconstruction clearly show the shape and structure of the midline of the brain and adjacent relationship. The average volume of the third ventricle of male is(1170.03 + 148.04)mm3,(854.64 + 43.59) mm3 for women, P>0.05,no significant difference between men and women. 3.The measurement results. The P value of P5-FN, P7-FN, AC-OC, and FB-AC were greater than 0.05. There was no difference between male and female. The P value of FN-AI was less than 0.05, and there have difference between male and female.The mean values of P5-FN of the male is(47.12±7.25)mm, the female is(46.83±5.14)mm; the mean value of P7-FN of the male is(48.95±7.02)mm,the female is(48.73±5.70) mm; the mean value of AC-OC of male is(10.78±1.15) mm,the female is(10.47±1.29) mm; the mean value of FN-AI of male is(7.42±1.03) mm,the female is(6.32±1.31) mm; the mean value of FB-AC of male is(4.51±0.85) mm,the female is(4.08±0.65) mm. The each mean values of P5-FN, P7-FN, AC-OC and FB-AC(Don't distinguish men and women) is(46.99±6.24)mm,(48.85±6.31)mm,(10.64±1.20)mm?(4.31±0.78)mm.Conclusion 1. We revealed the position, morphology and the relationship of each other through the celloidin slices of cerebral and the 3D reconstruction which provide morphological evidence for the resection of the deep tumor which located in cerebral and also provide important anatomical materials for the research of imaging.2. The third ventricle is used as the operation space of transcallosal approach. Therefore, it is important to measure the difference of the third ventricle volume between the male and female.3.On the basis of 3D reconstruction and transcallosal surgical approaches measure the distance of the P5-FN,P7-FN,AC-OC,FN-AI,FB-AC and compare the difference of male and female. Not only provide morphological support but also provide quantitative indicators for surgical treatment, at the same time, enrich the anatomical data of surgical procedures. |
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