Research Of Relationship Between Secretory Phospholipase A2 And Psoriasis Skin Lesion

Objective and contents:SPLA2-?A is one of the key members of the super family of Phospholipase A2, which associated with the pathogenic mechanism of various inflammation diseases, such as allergy, acute lung injury, pulmonary diffuse interstitial fibrosis, brain damage, arthritis, bone resorption and autoimmune cerebrospinal meningitis, etc. However, the molecular link between SPLA2-?A and psoriasis remains elusive. The aim of this study was to explore the relationship between the changes of the expression of sPLA2-?A and psoriasis skin lesions disease, to test the possibility of SPLA2-?A as a target molecule of psoriasis, providing experimental basis for the clinic early diagnosis and therapy of psoriasis disease.Through the questionnaire of genetic history and history of drug susceptibility, we collected the clinical information of related cases. Using immunohistochemical method, we evaluated the expression of sPLA2-?A in the skin tissue from the psoriatic patients and the control group. We also evaluated the correlation between the expression of sPLA2-?A in psoriatic patients and the severity of psoriasis. In addition, we detected the activity of PLA2-?A in peripheral blood collected from the research object. Furthermore, we detected the expression of sPLA2-?A mRNA in the skin tissues by RT-PCR. Taken together, these data revealed the interrelationship between the changes of expression of sPLA2-?A and psoriasis skin lesions. For guiding the early clinical diagnosis and treatment of psoriasis, we are making an effect to establish a more sensitive method to detect sPLA2-?A in the perspective of serology and gene level. Methods:Psoriasis specimens were detected from patients with typical skin lesions of the trunk or limbs, and non-psoriasis specimens were taken the patients' normal skin from the body or parts of the limbs as the control group(which was obtained from our hospital surgery and orthopedic surgery). Each specimen is sectioned and drop-fixed in a 10% formalin solution for histopathologic examination. For reminding part of the tissue, we store them in liquid nitrogen immediately for extracting total RNA. Using immunohistochemical method, we detect the expression of PLA2-?A in the psoriasis skin lesions, taken healthy skin as the control group. We also tested the activity of sPLA2-?A in peripheral blood of research objects via serological method. We further detect the mRNA level of sPLA2-?A in skin tissue of research objects using RT-PCR. Results:Immunohistochemical evaluation demonstrated that the expression of sPLA2-?A in psoriasis skin tissue was much higher than that of the normal skin tissues. ELISA analysis displayed much higher activity of sPLA2-?A in peripheral blood of research objects compared to controls. Using RT-PCR, we measured much higher mRNA levels for sPLA2-?A in patients with psoriasis skin tissue. Conclusions:sPLA2-?A is one of the key members of the family of PLA2, which plays an important role for the etiology of psoriasis, selective inhibition of sPLA2-?A can cure psoriasis. Among the 11 subsets of sPLA2, sPLA2-?A has a close relationship with psoriasis skin lesions. Our study showed that sPLA2-?A strongly expressed in upper epidermis psoriasis skin by immunohistochemical method, consistent with abnormal differentiation of keratinocyte cells. ELISA analysis also revealed a much higher level of sPLA2-?A in peripheral blood of psoriasis patients. Furthermore, we found that the expression of sPLA2-?A mRNA was higher than the control group skin. Therefore, we conclude that sPLA2-?A is a specific molecular target related to the pathogenesis of psoriasis and might be a new target point of psoriasis for molecular diagnosis and clinical therapy.