Metabolism Research On Different Surface Modification Of Gold Nanorods To Mice

Due to the rapid development of nanotechnology nowadays, more and more manufactured nanomaterials have been applied in every field of society. They are being used in electronics, tires, fuel cells and diagnostic assays in vitro, localized imaging, drug delivery and therapy in vivo relying on its unique physicochemical properties. Among them, gold nano-materials by has obvious surface effect, quantum effect, small size effect and biological compatibility for optical, elctronic, catalytic and biological medicine research and application hotspot. At present, in addition to the spherical gold nanoparticals, gold nanorods(Au NRs) have been widely studied and are becoming one of the most promising candidate for biomedical applications such as biosensors, cancer cell imaging, molecular detection, diagnosis, drug delivery and probes. However, the biological effect of Au NRs, namely, uptake, biodistribution, and storage in tissues in vivo, is closely relative to its own aspect ratio, surface charges, shapes, and increasing surface area relative to valume. When Au NRs interact with cell or protein, physical-chemical interactions between the NP surface and proteins can form a new interface, the so-called bio-nanointerface, and this will lead to the change of physico-chemical properties. As a consequence, when NPs into the blood by intravenous injection, the protein components in complex blood institutes compete to form a protein layer. NPs could be coated by high concentration and high-recombination-rate contact of protein, and forming a “protein corona”. The formation of protein corona would change the size and surface composition of Au NRs, which affect the absorption, transport and toxicity. To accurately know the metabolism and toxicity of Au NRs in vivo, gold nanorods with four different surface treats, including CTAB-Au NR, MS-Au NR, IMS-Au NR and MSA-Au NR were chosed, and used CD-1 mice as a model, and then observed the changes of liver, spleen, kidney tissues morphological and ALT, AST, TBil, TP, ALB, GLOB, GLU, GGT, ALP, TBA biochemical indexes by tail intravenous injection at 1, 3, 7 and 28 day points, investigate their uptake, biodistribution, retention, and toxic mechanism in vivo. The results showed that about 70% of the Au NRs in mice liver, liver and spleen are important organs of Au NRs distribution and metabolism. Enriched Au NRs can produce damage to the liver, liver injury of CTAB-Au NR group is more serious, and its toxicity mainly comes from CTAB, but “protein corona” nanorods have good biological compatibility. These nanoparticles are located in lysosomes of Kupffer cells in liver, and in the early stage caused lipid peroxide(known by MDA increase), and relying on the antioxidant system in the body to make it back to normal.