| Objectives This study was to explore the relationship between MCP-1, SAA and cognitive function in type 2 diabetes mellitus(T2DM) patients by detecting the levels of serum monocyte chemotactic protein-1(MCP-1) and serum amyloid A(SAA).Methods 1 subject Selected 116 T2 DM patients of Tangshan Gongren Hospital, 55 cases of male, 61 cases of female. All enrolled subjects received MMSE rating scale, according to MMSE score, they were divided into T2 DM cognitive impairment group of 50 cases, 24 males, 26 females and T2 DM cognitive normal group of 66 cases, 31 males and 35 females,age of two groups was matched. Then the subjects were divided into high-MCP-1 and lowMCP-1 groups, according to whether MCP-1 level was greater than its mean value.Similarly, they were divided into high-SAA and low-SAA groups. 2 methods Extracted elbow venous blood of all subjects. Detected clinical indicators, such as FPG, Hb A1 c, TC,TG, HDL, LDL, subjects received a RBANS scale test on the same day. Basic condition questionnaire was established to collect information of the subjects, including: sex, age,education level, duration, body mass index(BMI). Serum levels of MCP-1 and SAA were detected by enzyme linked immunosorbent assay(ELISA), the data above was analysed by SPSS 17.0.Results 1 Comparision betwwen T2 DM cognitive impairment group and cognitively normal group(1) comparison of demographic data and clinical and biochemical indexes:compared with cognitively normal group,the level of age,BMI,LDL, FPG, Hb A1 c, CRP in cognitive impairment group were higher, while the level of HDL was lower,the difference was statistically significant(P<0.05). The remaining indicators had no statistical differences(P>0.05).(2) Comparison of serum MCP-1, SAA: compared with cognitively normal group,the level of MCP-1, SAA in cognitive impairment group were higher, the difference was statistically significant(P<0.05). After the analysis of covariance for correcting the affection of basic information differences, serum MCP-1, SAA level were still significant difference between the two group(P<0.05).(3) Comparison of RBANS:compared with cognitive normal group, the scores of RBANS sections were all lower in cognitive impairment group, the difference was statistically significant(P<0.05). After the analysis of covariance for correcting the affection of basic information differences, the scores of RBANS sections were still all significant difference between the two group(P<0.05). 2 Comparison of high-MCP-1 and low-MCP-1 groups: the scores of immediate memory, visual span, language function, attention, standardized scores in low-MCP-1group were higher than in high-MCP-1 group, the difference was statistically significant(P<0.05), the scores of delayed memory score was no significant difference between the two groups(P> 0.05). 3 Comparison of high-SAA and low-SAA groups: the scores of attention, standardized scores in low-SAA group were higher than in high-SAA group, the difference was statistically significant(P<0.05), the scores of immediate memory, visual span, language function, delayed memory score were no significant differences between the two groups(P>0.05). 4 Correlation analysis of all the data subjects(1) the correlation of MCP-1 and basic information: pearson correlation analysis showed that serum MCP-1levels was positively correlated with SAA, Hb A1 c, FPG, CRP, and was negatively correlated with HDL(P<0.05), after the correcting of multiple stepwise regression analysis,MCP-1 was still positively correlated with SAA, Hb A1c(P<0.05).(2) the correlation of SAA and basic information: pearson correlation analysis showed that serum SAA levels was positively correlated with MCP-1?FPG ?TC?Hb A1 c ?LDL?CRP(P <0.05),after the correcting of multiple stepwise regression analysis, SAA was still positively correlated with MCP-1?FPG ?TC(P<0.05).(3) the correlation of MCP-1 and RBANS:pearson correlation analysis showed that serum MCP-1 levels was negatively correlated with immediate memory, visual span, language function, attention, delay memory and standardized scores(P<0.05), after the correcting of multiple stepwise regression analysis,MCP-1 was still negatively correlated with language function, attention, delay memory and standardized scores(P<0.05).(4) the correlation of SAA and RBANS: pearson correlation analysis showed that serum SAA levels was negatively correlated with immediate memory,language function, attention, delay memory and standardized scores(P<0.05), after the correcting of multiple stepwise regression analysis, MCP-1 was still negatively correlated with immediate memory, standardized scores(P<0.05).Conclusions 1 Serum MCP-1, SAA levels were significantly increased in T2 DM with impaired cognition. 2 Serum MCP-1, SAA levels were negatively correlated with cognitive function in T2 DM patients. 3 MCP-1, SAA may participate for the occurrence and development of T2 DM cognitive impairment as an independent risk factor respectively. |
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