The Role Of MiR-193b In The Migration And Invasion Of Human Glioma Cells

Background:Glioma occurs in the neural epithelium, the incidence rate, disability rate, postoperative recurrence rate and the highest mortality rate are the highest in the intracranial tumors, the incidence rate of adult is 6/10 million, 5 year survival rate is 20% ~ 30%. Surgical treatment is the most direct and basic treatment of gliomas, and it is also the first choice for the treatment of gliomas., but due to the invasive growth of glioma,and there is no obvious boundary with the normal brain tissue, so it is difficult to completely remove the tumor, and easy recurrence. So combination chemotherapy, radiotherapy and interventional therapy combined with chemotherapy, radiotherapy and interventional therapy are often used in clinical treatment., even so, its therapeutic effect is still poor, and the cure rate is low, the recurrence rate is high, The median survival time in patients with highly malignant glioblastoma is still less than 15 months. A large number of literatures have reported that mi RNA is closely related with the development of glioma, and it is expected to provide diagnostic markers and specific therapeutic targets for the clinical diagnosis of glioma.The research report points out that the abnormal expression of miR-193 b is closely related to the development of human malignant tumor.Mir-193 b plays in different tumors have different functions, it can promotes the development of bone mesenchymal stem cells, breast cancer, and head and neck squamous cell carcinoma,but also can inhibits the development of hepatocellular carcinoma, malignant melanoma, pancreatic cancer, esophageal cancer.However whether miR-193 b is associated with glioma? Whether to participate in the migration and invasion of glioma?Whether through the adjustment of MCT7 to achieve? These questions are not clear, therefore, the role and mechanism of mi R-193 b in gliomas is still need to be explored further. Objectives:To explore the effects and the underlying mechanisms of mi R-193 b in glioma cell migration and invasion,laying a foundation for formulating novel therapeutic target for treatment of glioma. Methods:Transient transfection miR-193 b mimics/NC to up-expressed the expression of miR-193 b in U251 cells by HiPerFect Transfection Reagent.To detect the transfection efficiency of miR-193 b by real-time PCR.The migration and invasion abilities were detected by Wound-healing assay and Transwell experiment in U251 cells respectively.Bioinformatics software was used to predict downstream target genes of miR-193 b.The Luciferase report gene identify the directly regulating target genes of miR-193 b, and using real-time PCR and Western blot experiment validated on mRNA and protein levels. Results:The expression of mi R-193 b in U251 cells increased significantly on mRNA levels after transient transfection,The Minics group was significantly higher than that of NC group and Mock group, the difference was statistically significant(P<0.05).Overexpression of miR-200 a promoted migration and invasion ability in U251 cells lines by Wound-healing assay and Transwell experiment(P<0.05).The bioinformatics software analysis showed that MCT7 genes maybe the target genes of miR-193 b,Using qRT-PCR, Western blot and luciferase report gene experiments further confirmed MCT7 are direct regulated downstream target genes of miR-193 b. Conclusions:1.Overexpression of miR-193 b can inhibit the migration and invasion of U251 cells.2.miR-193 b can down regulate the expression of MCT7 gene.3.miR-193 b may be a negative regulation of MCT7 gene to inhibit the migration and invasion of U251 cells.