Study Of The Determination Method Of The Content Of The Effective Components In Several Kinds Of Chinese Medicine Preparations By HPLC

In Chapter 1 ReviewIn the dissertation the index ingredients of Lingyang Qingfei Powder, Shufeng Sanre Capsule, Tongxuan Lifei Oral Liquid, Xiaoer Ganmaoning Syrup were selected as study object. The compound preparations quality were controled by High performance liquid chromatography, and in each study committed systematic methodology verification. And optimized the extraction process of Shufeng Sanre Capsule and Tongxuan Lifei Oral Liquid by response surface methodology. Meanwhile, Xiaoer Ganmaoning Syrup has been studied by HPLC with sample’ s internal standard. All these studies aim to provide a scientific reference for quality control of Chinese medicine preparation. This thesis is divided into the following five chapters: In Chapter 2 Simultaneous determination of the four effective components in Lingyang Qingfei Powder by double wavelength HPLCTo set up a method of determining the content of chlorogenic acid, gardenoside, paeoniflorin and liquiritin in Lingyang Qingfei powder. A double wavelength HPLC method was developed. The analysis was performed on a Kromasil C18 column（4.6 mm × 250 mm, 5 μm） with the mobile phase composed of acetonitrile and 0.1% phosphoric acid solution in gradient elution（0 6 min, 18% 24%A; 6 12 min, 24% 35%A）. The detector wavelength: 327 nm（chlorogenic acid）, 237 nm（gardenoside, paeoniflorin and liquiritin）.The flow rate was set at 1.0 m L·min^-1. The column temperature was 28 ℃. The linear ranges of chlorogenic acid, gardenoside, paeoniflorin and liquiritin were 1.579-78.96 μg（r = 0.9998）, 1.261-63.04 μg（r = 1）, 0.364-18.2 μg（r = 0.9999）, 0.3296-16.48 μg（r = 0.9999）, respectively. The average recoveries were 97.6%, 97.6%, 97.1% and 99.8%, respectively. RSDs were 0.97%, 0.73%, 1.2% and 1.3%, respectively. In Chapter 3 Determination of chlorogenic acid, geniposide, liquiritin and arctiin in Shufeng Sanre Capsule by HPLCAn effective method has been developed for simultaneous determination of chlorogenic acid, geniposide, liquiritin and arctiin in Shufeng Sanre capsule. Meanwhile, response surface methodology（RSM） is applied to predict optimum conditions for extraction. The method was performed on an Kromasil C18（250 mm × 4.6 mm, 5μm）. The mobile phase was composed of acetonitrile（A） – 0.1% phosphoric acid（B） with gradient elution（0 6 min, 20%A→24%A; 6 9 min, 24%A→30%A; 9 16 min, 30%A→45%A） at a flow rate of 1.0 m L·min^-1. The detection wavelength were set at 327 nm for chlorogenic acid in 0 4.5 min, 239 nm for geniposide in 4.5 7.5 min, 276 nm for liquiritin in 7.5 9 min, 280 nm for arctiin in 9 16 min, respectively. The column temperature was at 28 ℃. The flow rate was 1.0 m L·min^-1. These ingredients have higher linearity in 1.241-124.1, 0.788-78.8, 0.131-13.1 and 0.6482-62.82 μg?m L-1, respectively. Recoveries measured of chlorogenic acid, geniposide, liquiritin and arctiin were 98.3%, 97.2%, 97.6% and 96.7%, respectively. In Chapter 4 Simultaneous determination of naringin, hesperidin and baicalin in Tongxuan Lifei Oral Liquid by HPLCThe study was aimed at develop an HPLC method for simultaneous determination of naringin, hesperidin and baicalin in Tongxuan Lifei Oral Liquid and their extraction process was optimized by response surface methodology（RSM）. The analysis was performed on a Kromasil C18 column（4.6 mm×250 mm, 5 μm）. The mobile phase consisted of acetonitrile（A） and 0.5% phosphoric acid（B） with gradient elution（0 7 min,26%A; 7 12 min, 26%⁷0%A）.The detection wavelength was set at 280 nm. The column temperature was kept at 28 ℃. The flow rate of analytic process was 1.0 m L·min^-1. There is a good linear relationship of naringin, hesperidin and baicalin within 6.976-174.4 μg·m L-1（r = 0.9999）, 3.533-88.32 μg·m L-1（r = 0.9999）, 1.434-35.84 μg·m L-1（r = 0.9999）. The average recoveries of those were 98.4%, 98.3% and 98.6%, respectively. RSDs were 1.0%, 0.67% and 0.74%, respectively. In Chapter 5 Determination of chlorogenic acid,geniposide,baicalin and arctiin in Xiaoer Ganmaoning Syrup by HPLC with sample’ s internal standardTo establish a HPLC method with sample’s internal standard（SIS） for simultaneous determination of chlorogenic acid, geniposide, baicalin and arctiin in Xiaoer Ganmaoning Syrup. Geniposide in Xiaoer Ganmaoning Syrup was chosen as the internal standard, and the related correction factors of chlorogenic,acid baicalin and arctiin compared with geniposide were measured. The contents of these three components could be calculated by the related correction factors.This method was named to be sample’s internal standard. The veracity and scientificity was evaluated by comparison of the quantitative results between angle cosine value（ACV） and SIS method. The results show there were no significant differences in the quantitative results of the four components in three batchs of Xiaoer Ganmaoning Syrup by ACV and SIS methods（P>0.05）.