Ajuba Regulates Cell Migrationin And Cell Proliferation In Human Cancers

Objective To observe the effect of the interaction between LIM protein Ajuba and transcription factor Twist in colorectal cancer and to verify the affection on CRC cell migration ability. Methods Co-immunoprecipitation test was used to detect the interaction between Ajuba and Twist. The transcription activity level of N-cadherin promoter was estimated by luciferase report gene assay under the condition of exogenous Ajuba protein concentration gradient. Ajuba knock-down stable cell lines in SW1116 were established and the cell migration ability was tested by transwell assay. Results Exogenous Ajuba interacts with Twist, which can enhance the transcription activity of N-cadherin promoter, which is one of the classic target genes of Twist. When Ajuba were transfected in 293 T cell in the concentration of 50?100?200ng/well, the luciferase activities were up-regulated. Similarly, when Ajuba transfected company with Twist, the results of the luciferase activity increase two-fold. In SW1116-sh Ajuba cells, the cell migration abilities were repressed due to the down-regulation of Ajuba protein level. Conclusion LIM protein Ajuba is a new coactivator of transcription factor Twist, which can promote N-cadherin expression and enhance CRC cell migration ablity. Due to the high expression level of Ajuba protein in CRC, Ajuba may be a potential diagnostic marker to estimate the degree of tumor metastasis.Objective To observe the effect of the interaction between LIM protein Ajuba and nuclear receptor ER? in breast cancer cell T47 D and to verify the affection on Br CA cells' growth and prolifeartion. Methods Co-immunoprecipitation test was used to detect the interaction between Ajuba and ER?. The IF pictures show the co-location results of Ajuba and ER?. Stable expression cell lines were established, such as T47D-sh Ajuba,T47D-oe Ajuba and T47D-sh ER?, and their growth activity were also measured by CCK-8 tests. The transcription activity level of CDK6 promoter was estimated by luciferase report gene assay under the condition of exogenous Ajuba protein and ER?. Endogenus Ch IP assaies were performed in wild type T47 D cells to exam the enrichment of Ajuba and ER? on the potential ERE site of CDK6 promoter. Results Exogenous Ajuba interacts with ER?, which can enhance the growth activity of BrCA cell T47 D. When Ajuba were transfected in 293 T cell with ER?, the luciferase activities were up-regulated. Ajuba, company with ER?, can be recuried to the CDK6 promoter. Conclusion LIM protein Ajuba is a new coactivator of nuclear receptor ER?, which can promote CDK6 expression and enhance Br CA cell growth activity.