Effects Of Morindae Officinalis Radix Polysaccharide On FSHR Expression Of Testis Sertoli In Experimental Varicocele-induced Sprague-Dawley Rats

Objective:To investigate the effects of different concentration of MOP on spermatoge nesis in varicocele-induced adult male Sprague-Dawley(SD) rats.Methods:75 adult SD male rats were randomly divided into 5 groups: the sham operation group(normal group) 、 model group(normal saline group) 、 VC treatment with200mg/kg MOP group、VC treatment with 300mg/kg MOP group、VC treatment with400mg/kg MOP group. For 1 month after lagtion, operate treatment for 4 weeks, the body weight, the testis weight/body weight ratio and epididymis weight/body weight ratio were record. The morphological changes of testicular tissue were stained by HE and the follicle stimulating hormone receptor(FSHR) was located in Sertoli cells by immunohistochemistry which were observed under optical microscope, QPCR measure testis tissue FSHR m RNA expression level. Finally, the expression level of FSHR was detected by western-blotting.with MOP was prominently different from model group and normal group(P<0.05).3.In model group, the epithelium of seminiferous tubule was thinned,spermatogenic cellular level in the lumen were disordered, each level of spermatogenic cells is a significantly sparse distribution, spermatoblast and sperms could not be seen in the lumen, but necrotic cells; the situation was improved in three treatment groups with MOP, is that, it could be seen that the structure of seminiferous tubule was basically normal with thickened seminiferous epithelium and hierarchical spermatogenic cells in the lumen, the increasing number of spermatogenic cells in each level and a close arrangement, and there are many sperms could be seen in the lumen.but,It did not return to normal levels.2.The testis weight/body weight ratio: They were significant difference compare with model group and normal group, sham operation group was dramatically higerer(P < 0.05); the testis weight/body weight ratio of treatment groups with MOP was dramatically higerer than model group(P<0.05). the epididymis weight/body weight ratio: They were significant difference compare with model group and normal group,sham operation group was dramatically higerer(P<0.05); compare with model group and normal group,While The epididymis weight/body weight ratio of treatment groups Results:1.After treatment with MOP, the body weight of model group was not significant difference compared with sham operation group(p>0.05); and that of model group was not significantly different from the other treatment groups with MOP(p>0.05).4.The results of immunohistochemistry showed that FSHR was mainly expressed in the peripheral Sertoli cells of the testicular seminiferous tubule in testicular tissue.5.QPCR analysis of FSHR m RNA showed the expression of FSHR m RNA level was significantly increased in three treatment groups with MOP,compared with model group(P<0.05), and was highest in VC treatment with 300mg/kg MOP group.6.Western Blot analysis of FSHR protein showed that the expression of FSHR protein level was significantly increased in in three treatment groups with MOP(P<0.05), and was highest in VC treatment with 300mg/kg MOP group.Conclusion:MOP improve the expression of FSHR m RNA and FSHR protein on Sertoli cells which isolated from the injured spermatogenic functions of the rats, The testis weight/body weight ratio and he epididymis weight/body weight ratio were dramatically higerer, the epithelium of seminiferous tubule was fixed and leads to improve the functions, so as to recover and promote spermatogenesis.