The Effects Of Human Mesenchymal Stem Cells On Angiogenesis Of HCC877 Lung Xenograft Tumor

Objective:(1)To study the tumor homing properties of human Mesenchymal stem cel ls(h BMSCs) to the subcutaneous transplanted HCC827 human pulmonary adenoca rcinaoma model in naked mouse through tail vein.(2)To lern the effects of low-dose metronomic cis-platin(DDP) chemothera py and h BMSCs on the growth of xenograft tumor.(3)To learn the metronomic chemotherapy and h BMSCs on angiogengesis of subcutaneous xenograft tumor.Methods: To establish the subcutaneous xenograft tumor model of human HCC827 lun g carcionma in 40 healthy female Balba/c nude mouse, divide them into four gro ups randomly, every grough has 10 mice. The mice received the low-dose metron omic cis-platin chemotherapy through intraperitoneal injection at the 22 st day, the h BMSCs labeled with Brd U thro ugh tail vein. The cell surface antigens of h BM SCs detected by flow cytometry. The tumor paraffin sections detected in imunohi stochemistry to observe the expression of VEGF and the microvessel density(MV D) of the tumor; The protein concentration of human HIF-1? and IL-6 in the tu mor tissue detected in the experimental of enzyme linked immunosorbent assay(EL ISA).Results: We can see that the expression of Brd U always appear around the vessels i nthe stromal of xenograft tumor. Compared with the contro l group, h MSCs have no obvious effects on the growth of HCC827 xenograft tumor(P>0.05), but the DDP group or combing group have the significant inhibitory effect on the tumor (P<0.05).We have not find the ditant matastases among lung?liver or spleen.The MVD decreased after 7 days in the treatment of DDP, on the 14 th,MVD and th e expression of VEGF in the DDP group increased obvisouly. But the group with the h BMSCs have the lower expression of MVD and VEGF(P<0.05),at the same time IL-6?HIF-1? and VEGF which detected by ELISA decreased(P<0.05).Conclusion: The h BMSCs can homing to the subcutaneous xenograft tumor by tail vein and supress the angiogenesis, but they have no effects on the growth of HCC827 lung carcinoma xenograft tumor. In the group of DDP, the growth of tumor wa s depressed obviously, combined DDP and h BMSCs, maybe through the down-reg ulate the expression of VEGF, HIF-1? and IL-6, h BMSCs can modify the vescula ture andsupress the increasing of vessels, alleviate the hopoxia.