| Objectivewe made the terror stress in adult female rats by adopting on-looking electric shock on pregnant rats, and then we evaluated the model by the female ethology score and the secretion of ACTH, GC index. Meanwhile, the ethology scores of the rat's offspring with different-day old were observed and the secretions of monoamines, amino acids transmitter outside the hippocampus cells of the offspring were detectedMethodsSPF animal:34 female rats,80-day-old, weight 220-250g; 25 male rats, 80-day-old, weight 250-290g. We evaluted emotional and cognitive of the aninmal through maze water and so on, and choosed the female rats whose emotional and cognitive were at the same level, then divided them into control group and model group by the random number table with 15 rats in each group, and extra females on standby. Chosed 15 male rats whose emotional and cognitive were at the same level. On the first day, the terror stress in adult female rats was made by on-looking electric shock, and then observed the model and tested the index:(1) Behaviors of the pregnant rats were detected by the open field test, sucrose preference test and tail suspension test every 6 days. (2) Morris water maze was adopted on the 16th day of fertilization. (3) The content of blood ACTH and GC from orbit was detected on the 20th day. All above mentioned were used to evaluate the terror stress in adult female rats model. After the offspring were born,5 young rats were chosen by the random number table to be observed and detected the relevant indexes:(1) the general developmental capacity including eye open, clothing hair and ear perk. (2) Neurodevelopment including sensation reflex, acoustic startle reflex (ASR) and plantar grasp reflex. (3) The 21-day-old and the 80-day-old offspring rats were given the open field test, sucrose preference test, tail suspension test and Morris water maze to evaluate the emotion and the cognitive function on both groups. (4) Detected the monoamines such as L-DOPA, DA, HVA, EP,5-HT and the amino acids transmitter such as GLU, GABA, GLY in hippocampus cells of the 21-day-age and the 80-day-age offspring rats by microdialysis and high performance liquid chromatography (HPLC).Results1. Building the terror stress in adult female rats during pregnancy:(1) In the open-field test, the rearing score, the crossing score and number of cleaning cycles of the female rats in model group were less than that of those in the control group, while the dejection amounts were on the contrary significantly (P<0.05), the modeling time of the number of cleaning cycles and the dejection amounts had interaction effect with the stimulating factors, but there was no interaction effect between the modeling time of the rearing score and the crossing score with the stimulating factors. (2) In the sucrose preference test, level of sucrose preference of model group decreased steadily as the modeling prolonged significantly (P<0.05). (3) In the tail suspension test, the impending still time of the pregnant rats of model group increased as modeling time prolonged compared with the pregnant rats of the control group significantly (P<0.05). And the impending still time had interaction with the stimulating factor of the pregnant rats of each group. (4) In the Morris water maze, on the 1st,2nd and 3rd modeling day, the mean escape latency of the model group was prolonged compared with the pregnant rats of the control group significantly (P<0.05). In the model group, the swimming speed slowed down, times of run across platform decreased and time of duration of stay at 20% sidelines increased compared with the pregnant rats of the control group, these all had statical differences (P<0.05). (5) Level of ACTH and GC of pregnant rats of the model group were higher than pregnant rats of the control group, these all had statical differences (P<0.05).2. Compare the general condition and neurodevelopment of young rats: Time of tooth eruption, clothing hair, emerging of sensation reflex, placing reflex of limbs, negative geotaxis and gait reflex of the model group were late than these of the control group significantly (P<0.05). Time of ear perk, eye open, turn over, ASR and plantar grasp reflex had no significant difference in each group (P>0.05).3. Compare the ethology of the 21-day-old and the 80-day-old young rats:(1) The open-field test:the rearing score and the crossing score of the 21-day-old and the 80-day-old offspring rats in model group were less than that in the control group significantly (P<0.05). The dejection amounts and number of cleaning cycles of the offspring rats had no significant difference with the offspring rats of the control group (P> 0.05). (2) The sucrose preference test:sugar water consumption and the level of sucrose preference of the 21-day-old and the 80-day-old offspring rats in model group decreased compared with the offspring rats in control group. (3) The tail suspension test:the impending still time of the offspring rats of model group was longer than the offspring rats in the control group significantly (P<0.05). (4) The Morris water maze:the mean escape latency of the offspring rats in the control group was shorter than the offspring rats in the model group significantly (P<0.05). In the control group, time of duration of stay at 20% sidelines increased, the swimming speed was faster, times of run across platform were more often than that of the offspring rats of the model group significantly (P<0.05).4. Compare the ACTH, GC of the 21-day-old and the 80-day-old youngof rats:level of ACTH and GC of offspring rats in the model group were higher than that of offspring rats in the control group.5. Compare the secretion of monoamines of hippocampus cells of the offspring rats. (1) level of L-DOPA and 5-HT in the hippocampus cells of each perfusion time point of the 21-day-old offspring rats in the model group were lower than the control group (P<0.05). While the level of the two of the 80-old-age offspring rats between the model group and the control group had no significant difference (P> 0.05). Level of NE, EP, DOPAC, DA,5-HIAA and HVA in the hippocampus cells of each perfusion time point of the 21-day-old and the 80-day-old offspring rats in the model group were lower than the control group (P<0.05).6. Detection of amino acids transmitter in the hippocampus cells of offsring rats. (1) Level of GLU, GLY in the hippocampus cells of each perfusion time point of the 21-day-old and the 80-day-old offspring rats in the model group were higher than the control group, the GABA was in contrast, these had significant statical differences(P<0.05); (2) Level of ASP in the hippocampus cells of each perfusion time point of the 21-day-old offspring rats in the model group were higher than the control group, and had statical difference (P<0.05), while there had no significant difference between the model group and the control group at the age of 80 (P> 0.05).7. Correlations:level of GLU and GLY in the hippocampus cells of each perfusion time point of the 21-day-old and the 80-day-old offspring rats in the model group were higher than the control group significantly (P <0.05). While level of GABA was on the contrary (P<0.05). Level of ASP outside the hippocampus cells of each perfusion time point of the 21-day-old offspring rats is higher than the offspring rats in the control group significantly (P<0.05). There is no significant difference with the 80-day-old offspring rats on the level of ASP outside the hippocampus cells of each perfusion time point in both groups (P>0.05).Conclusion1. Making the terror stress in adult female rats model during pregnancy is successful. The model group showed high stressful state in terms of the ethology and the secretion of ACTH, GC.2. The terror stress in adult female rats during pregnancy do have effect on both the emotion and short-long term cognitive development of the offspring rats.3. The emotion and cognitive development of the offspring rats has a close correlation with the amount of monoamines and amino acids transmitter outside the hippocampus cells. |
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