A Study Of Detection Methods Of High-Risk Human Papillomavirus

Human papillomavirus (HPV) is a DNA virus, closely related to the occurrence of major human skin and mucous membrane infections, persistent high-risk HPV infection and cervical cancer, thus establishing a quick and easy detection of high-risk HPV types the method has very important significance. The main contents of this study are as follows:The first chapter, a brief introduction of the human papillomavirus and its relationship to cervical cancer, HPV detection methods are summarized and elaborated on some of the nucleic acid isothermal amplification method and its application, we put forward research ideas and methods of the subject.The second chapter, a high-risk human papillomavirus HPV16 and HPV 18 detection method based on the polymerase chain reaction and microchip electrophoresis was established, respectively, using the universal primers and specific primers for amplification of HPV DNA can be detected in order to achieve content, DNA sequencing PCR products confirmed the accuracy of the two amplified. Microchip electrophoresis system using the PCR product to achieve a rapid high-throughput DNA analysis of samples, single sample detection time is about 2-3 minutes, at least sample consumption of about 2?L, sensitivity up to 102 cells.The third chapter, a high-risk human papillomavirus HPV16 and HPV18 detection method based on the loop-mediated isothermal amplification (LAMP) visualization was established, the product can be used to detect visually. Compared with traditional methods, all reactants in advance plus a good, the whole reaction process without opening the lid, no extra added after the end of a fluorescent dye, to avoid cross-contamination may have caused, while greatly reducing the reaction time and increase the reaction accuracy of early screening of cervical cancer are expected to be used to assist with the practical application.