Effect Of Saikosaponin A On The Proliferation Of HaCaT Cells

Objective: To observe the effect of Saikosaponin a on the proliferation of HaCaT cells, and to provide theoretical basis for the treatment of psoriasis vulgaris with Saikosaponin a.Methods: Ha CaT cells were cultured and seeded on 96 well plates,0ug/ml, 25.0ug/ml, 50.0ug/ml, 75.0ug/ml, 100.0ug/ml, a, respectively, were intervened by,After 24,48,72 hours, the survival rate of cells was calculated and the cell proliferation curves were drawn.Results:Saikosaponin a on HaCaT cells morphological effects on HaCaT cells of saikosaponin a 24 h after, Oug/ml group and 25.0ug/ml group on the cell shape and size without significant changes, showed only a decrease of adhesion, exfoliated cells and floating; 50.0ug/ml group cell volume slightly variable small granules within the cell vacuoles increased, cell adhesion variation, more cells shed from the bottle wall, floating in the culture medium; group 75.0ug/ml, 100.0ug/ml group the cells or all off, floating.25.0ug/ml saikosaponin a on HaCaT cell survival rate of the 24 h for 81.62%, 48 h for 66.84%, 72%, 50.0ug/ml saikosaponin a on Ha CaT cell survival rate of the 24 h 38.46%, 48 h for 16.05%, 72 hours for 11.52%, 75.0ug/ml saikosaponin a on HaCaT cell survival rate of the 24 h 14.96%, 48 h 7.58%, 72 h was 2.19%, 1 00.0ug/ml HaCa T cell survival rate of 24 h was 2.35%, 48 h was 1.06%, 72 h was 0.73%.Conclusion: the effect of Saikosaponin a on the proliferation of HaCa T cells, and the survival rate of HaCaT cells gradually decreased with the increasing time of a.