MiR-200c Inhibits Migration And Invasion In Human Gastric Cancer SGC7901 Cells By Downregulating DCLK1

Objective:To observe the effect of miR-200 c on migration and invasion in human gastric cancer SGC-7901 cells,and to find out the possible mechanism.Methods:Human gastric cancer SGC-7901 cells were transfected by lentiviral vector with miR-200 c overexpression(miR-200 c group) and empty lentiviral vector. Empty lentiviral vector transfection group was taken as an negative control group, and a group that was not transtected was blank control group. After 72 h of transfection, the transfection efficiency was observed under a fluorescence microscope. Wound healing and transwell assays were performed to detect the effect of miR-200 c on SGC-7901 cells migration and invasion. RT-PCR and Western blot analysis were used to detect the epression level of possible target gene DCLK1.Results:After 72 h of transfection, the cells were observed and counted under a fluorescence microscope, and the efficiency of miR-200 c expression with lentiviral vector and negative control lentiviral transfecting human gastric cancer SGC-7901 cells were above 90%. Wound healing showed that the scar distance of miR-200 c group was wider than negative control group and blank control group at 8h and 24h(P<0.05). No difference was found between negative control group and blank control group(P>0.05). Transwell assays showed that the number of cancer cells which passed through the matrigel coated membrance was obviously reduced in miR-200 c group than in negative control group and blank control group(P<0.05). No difference was found between negative control group and blank control group(P>0.05). It showed that after miR-200 c was transfectioned into SGC-7901 cells, the migration and invasion of SGC-7901 cells were significantly suppressed(P<0.05). Bioinformatics predicted that DCLK1 may be a target gene of miR-200 c. RT-PCR and Western blot showed that the expression levels of DCLK1 mRNA and protein were significantly lower in the lentivirus infected SGC-7901 cells with miR-200 c overexpression(P<0.05). The expression levels of DCLK1 were no difference between negative control group and blank control group of SGC-7901 gastric carcinoma cells(P>0.05).Conclusion:miR-200 c can inhibit the migration and invasion of SGC-7901 gastric cancer cell by downregulating the expression of DCLK1.