The Changement Of Mitochondrial Coupling Factor 6 And Cytochrome C And Their Clinical Significance In Neonatal Sepsis

Objective:To evaluate the changement of the plasma level of coupling factor 6(CF6) and cytochrome C(Cyt-c) in neonatal septicemia.,and to explore the clinical significance in neonatal sepsis.Method: A total of 88 neonates admitted to Hunan Children's Hospital from January 2015 to April 2015 were collected. Femoral venous blood was collected in all patients before the use of antibiotics after admission. The levels of Cyt-c and CF6 in plasma were measured by enzyme-linked immunosorbent assay(ELISA). Basedion the patients' clinical data,routine blood test,CRP,PCT,ESR,,blood culture and other laboratory indicators were divided into non sepsis group(n=42) and sepsis group(n=46),The differences of clinical data between the two groups were compared.Meanwhile the non sepsis group was divided into non infection group and common infection group, and the sepsis group was divided into general sepsis group(no organ failure) and MODS group(combined with multiple organ failure).The changes of the level of CF6 and Cyt-c between these groups were compared.And the sensitivity and specificity differences with the traditional sepsis index(CRP, PCT) were analyzed. Compared together with the correlation of the neonatal critical illness score.SPSS18.0 was used to statistical analyze.Result:1.In sepsis group the level of CF6 were(109.7±8.9) pg/ml,and in non sepsis group the level of CF6 was(46.3±6.0) pg/ml, the difference was statistically significant(t=-6.32,p=0.00).In sepsis group the level of Cyt-c was(44.5±4.9) ng/ml,and in non sepis group was(31.8±6.7)ng/ml.The difference was statistically significant(t=-9.95,p=0.00).2. The ROC curve showed that the sensitivity and specificity of CF6 were 0.761,0.732, and the Cyt-c were 0.739,0.714.3. In non infection group?common infection group?general sepsis group and MODS group the level of CF6 were(32.1±8.9) pg/ml,(59.3±7.2)pg/ml,(79.3±5.9)pg/ml,(172.6±6.1)pg/ml respectively(F=51.6,p=0.00). The level of Cyt-c were(29.3±8.6)ng/ml,(35.4±4.1) ng/ml,(43.1±5.9) ng/ml,(44.5±5.9)ng/ml respectively(F=50.1,p=0.00). The differences between these groups were significant.4.Pearon's correlation of CF6 and neonatal critical illness score were r=-0.599, p=0.000;Cyt-c were r=-0.337,p=0.001.Cyt-C and CF6 were negatively correlated with the neonatal critical illness score.Conclusion:1. CF6 and Cyt-c level increased in neonatal sepsis.The damage of mitochondria may be one of the pathological mechanisms in neonatal sepsis.2. The levels of CF6 and Cyt-c were closely related to the severity of neonatal sepsis.