| Part 1 Investigation of Virus Spectrum of Hospitalized Children with Acute Low Respiratory Infection from 2012-2014 in ChangshaObjectiveDynamic monitoring of 12 kinds of respiratory viruses epidemic law of acute lower respiratory infections( ALRTI) in hospitalized children form 2012 to 2014 in Changsha area, for the prevention and control of the region’s ALRTI children accumulating epidemiological data.Subjects and Methods1. NPAs were collected from hospitalized children in Hunan Provincial People’s Hospital Children’s Medical Center with ALRTI from April 2012 to March 2014.2. Respiratory syncytial virus(RSV),human rhinovirus(HRV),influenza virus A(IFVA),influenza virus B(IFVB),parainfluenza 1-3(PIV1-3), Human metapneumovirus(HMPV), human coronaviruses HKU1(HCo V-HKU1), human coronaviruses NL63(HCo V-NL63),adenovirus(ADV) and human bocavirus(HBo V) of NPAs specimens were detected by real-time fluorescence quantitative polymerase chain reaction(RT-FQ PCR).3. Statisticing and analysing epidemiological characteristics of virus by checking the hospital clinical data of hospitalized children with ALRTI.Results1. Among the 1101 NPA,viruses were detected in 871 samples(87.4%),positive detection rate of RSV,PIV3 and HRV is higher, ADV and IFVA positive detection rate rise significantly compared with the early data.2. Detection rate of the male and female was no difference(χ~2=0.345,P=0.552)),in different age groups and season have statistical significance, of which 6 month to 1 year old of positive detection rate is higher(χ~2=69.956,P=0.000),the peak prevalence of viral was in winter and spring(χ~2=73.111,P=0.000).3. Virus co-detection rate is 35.6%, RSV,HBo V, HRV, PIV3, ADV is common to virus co-detection.4. Virus co-detection rate between different gender, age, season had statistical significance, men more than women(χ~2=4.527,P=0.033), mixed detection is more commonly under 3 years old(χ~2=11.684,P=0.020),winter and spring is more commonly than autumn and winter(χ~2=19.107,P=0.000).Conclusions:During the study period, virus spectrum and popular characteristics of ALRTI different with previous years in hospitalized children, mixed detection of virus is common.Part 2 Analysis of viral load in Children with Acute Low Respiratory Infection from 2012-2014 in ChangshaObjectiveUnderstanding common respiratory virus of distribution characteristics of viral load in hospitalized children with ALRTI form 2012 to 2014 in changsha region,compare the difference of viral load between pure and mixed detection, to explore its clinical significance.Subjects and Methods1. The object of study,the experimental material, experimental method, as the first part.2. To Statistical and analysis of positive cases of viral load distribution characteristics, and the viral load of difference between simple and mixed detection.Results1. No statistically significant difference between viral load of simple and mixed detection of HBo V(P=0.444),viral load of simple detection HBo V cases have differences in different age distribution(P = 0.007), while no statistical significance in different gender and seasonal distribution of the viral load of simple HBo V detection, viral load of mixed detection HBo V cases has differences in different age(P = 0.000) and seasonal(P = 0.018) distribution, but no difference between the sexes (P = 0.696), the HBo V viral load of simple HBo V detection higher Significantly than HBo V/RSV mixed detection(P = 0.035), but compared to HBo V/HRV(P = 0.928), HBo V/PIV3(P = 0.928), HBo V/ADV(P = 0.711), HBo V/HMPV(P = 0.979) there was no statistically significant.2. Mixed detection RSV cases of viral load significantly higher(P = 0.000) than simple detection,Pure and mixed detection RSV viral load cases are high for children under the age of 1 years old(P= 0.000), the winter is higher than the summer fall, but no differences between different gender;In addition to RSV/HCo V-HKU1(P = 0.900), were simple RSV/HBo V(P = 0.000), RSV/HRV(P = 0.000), RSV/IFVA(P = 0.000), RSV/PIV3(P = 0.000), RSV/ADV(P = 0.000), RSV/HMPV(P = 0.000), RSV/HCo V-NL63(P = 0.005) detection of RSV cases high viral load.3. The viral load of simple and mixed detection HRV cases no difference(P = 0.501),The difference in different gender, age and season distribution were not statistically significant,Simplicity and HRV/HBo V(P = 0.097), HRV/RSV(P = 0.182), HRV/PIV3(P = 0.325), HRV/ADV(P = 0.920) detection of HRV no statistically significant difference were observed in the viral load.4. The viral load of simple and mixed detection IFVA cases no difference(P = 0.917),The difference in different gender, age and season distribution were not statistically significant, Simplicity and IFVA/HBo V (P = 0.382), IFVA/RSV(P = 0.529), IFVA/PIV3(P = 0.493) mixed detection IFVA no statistically significant difference were observed in the viral load.5. Mixed detection PIV3 cases of viral load significantly higher(P = 0.000),The difference in different gender, age and season distribution were not statistically significant, Besides PIV3/HRV(P = 0.195), were simply PIV3/HBo V(P = 0.000), PIV3/RSV(P = 0.000), PIV3/IFVA(P = 0.029), PIV3/ADV(P = 0.003), PIV3/HMPV(P = 0.005),(PIV3/HCo V-NL63(P = 0.010) mixed detection PIV3 high viral load cases. Viral load of simple and mixed detection of HMPV are no difference in different gender, age, season, simple detection HMPV viral load is higher than HMPV/RSV(P = 0.000), HMPV/HCo V-HKU1 detection(P = 0.044), but with HMPV/HBo V, HMPV/PIV3, HMPV/ADV mixed detection is no difference.6. Mixed detection HMPV cases of viral load significantly higher(P = 0.000), the distribution of different gender, age, season have no difference, the viral load of simple detection is higher than HMPV/RSV(P = 0.000), HMPV/HCo V-HKU1(P = 0.044) detection, but with HMPV/HBo V(P = 0.062), HMPV/PIV3(P = 0.044), HMPV/ADV(P = 0.062) detection of HMPV viral load has no difference.7. Simlpe and mixed detected ADV cases of viral load have no difference(P = 0.368),The difference in different gender, age and season distribution were not statistically significant, simple ADV detection compare to ADV/HBo V(P = 0.953), ADV/RSV(P = 0.475), ADV/IFVA(P = 0.055), ADV and PIV3(P = 0.591), ADV/HMPV(P = 0.097) the viral load of ADV in comparative differences had no statistical significance.8. Mixed detection PIV2 cases of viral load significantly higher(P = 0.005), Viral load of simple and mixed detection HCo V-HKU1(P = 0.947), PIV1(P = 0.615) cases no difference, HCo V-NL63 simply check out only 1 case, IFVB are mixed checked out.ConclusionsIn this study,the viral load of distribution for different viruses have different characteristics, part of the mixed infection can make viral load disturbance. |
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